【摘要】：【目的】本研究旨在探究銼吸式口器害蟲西花薊馬Frankliniella occidentalis取食誘導(dǎo)的菜豆Phaseolus vulgaris木植株系統(tǒng)防御反應(yīng)的分子機(jī)制。【方法】利用熒光定量PCR技術(shù),檢測西花薊馬分別在穩(wěn)定取食菜豆植株2 h后的24,48,72和96 h菜豆植株不同部位(上部、中部和下部)葉片中茉莉酸信號轉(zhuǎn)導(dǎo)途徑和水楊酸信號轉(zhuǎn)導(dǎo)途徑防御相關(guān)基因脂氧合酶基因(LOX)、苯丙氨酸解氨酶基因(PAL)和β-1,3-葡聚糖酶基因(PR-2)相對表達(dá)量的變化�！窘Y(jié)果】西花薊馬取食菜豆植株后,受害葉片及上部和下部健康葉片中LOX,PAL和PR-2均被顯著誘導(dǎo)表達(dá),均以受害葉片防御酶基因表達(dá)量的變化較大,并且表達(dá)量在相同葉片不同時(shí)間下的變化不同。中部受害葉片中LOX的相對表達(dá)量均顯著高于未接蟲的對照植株,并隨西花薊馬為害時(shí)間的延長逐漸升高,在96 h時(shí)為對照的209.54倍;隨著時(shí)間的延長LOX表達(dá)量在上部和下部健康葉片呈現(xiàn)升高-降低-升高的趨勢。PAL的表達(dá)量在西花薊馬為害植株的中部受害葉片和上部健康葉片均于48 h時(shí)出現(xiàn)最大值,分別為對照的52.70倍和41.20倍;但在下部健康葉片于72 h時(shí)達(dá)到最大值,為對照的47.06倍。PR-2表達(dá)量在受害株的上部健康葉片于48 h時(shí)出現(xiàn)最大值,在中部受害葉片和下部健康葉片均于96 h時(shí)達(dá)到最大值,但在24 h時(shí)在下部健康葉片中受到抑制。【結(jié)論】西花薊馬取食能顯著誘導(dǎo)菜豆植株茉莉酸和水楊酸信號轉(zhuǎn)導(dǎo)途徑相關(guān)防御酶基因LOX,PAL和PR-2的表達(dá),并在菜豆植株不同部位葉片產(chǎn)生系統(tǒng)抗性。
[Abstract]:[objective] to explore the molecular mechanism of systemic defense response induced by Frankliniella occidentalis, a rasping mouthpiece pest, in Phaseolus vulgaris plantlets. [methods] fluorescence quantitative PCR technique was used. The different parts (upper part) of vegetable bean plant were detected at 244872 and 96 h after stable feeding for 2 h. Changes in the relative expression levels of Jasmonic Acid signal Transduction Pathway and Salicylic Acid signal Transduction Pathway Defense related Gene Lipoxygene-associated Gene (LOX), phenylalanine Ammonia-lyase Gene (PAL) and 尾 -1 3-glucanase Gene (PR-2) in the Middle and Lower part of the leaves. [ Results: after feeding on bean plants, thrips of western flowers, The expression of LOXPAL and PR-2 in the injured leaves and the upper and lower healthy leaves were significantly induced. The expression of defense enzyme genes in the injured leaves varied greatly, and the expression levels varied at different time points in the same leaves. The relative expression of LOX in the middle damaged leaves was significantly higher than that in the control plants without inoculation, and increased gradually with the prolongation of the damage time of the western flower thrips, and was 209.54 times of that of the control at 96 h. With the extension of time, the expression of LOX increased, decreased and increased in the upper and lower healthy leaves. The expression of pal reached the maximum at 48 h in the middle injured leaves and the upper healthy leaves of the plants damaged by thrips sibirica. The expression of PR-2 was 52.70 times and 41.20 times of the control, respectively, but reached the maximum at 72 h in the lower healthy leaves, and the maximum expression of PR-2 was 47.06 times at 48 h in the upper healthy leaves of the affected plants. The maximum value of damaged leaves and lower healthy leaves was reached at 96 h. But it was inhibited in the lower part of the leaves at 24 h. [conclusion] the feeding of thrips can significantly induce the expression of LOXPAL and PR-2 genes related to the signal transduction pathway of jasmonic acid and salicylic acid in bean plants. Phylogenetic resistance was produced in different parts of soybean leaves.
【作者單位】： 貴州大學(xué)昆蟲研究所貴州省山地農(nóng)業(yè)病蟲害重點(diǎn)實(shí)驗(yàn)室;
【基金】：國家自然科學(xué)基金項(xiàng)目(31160367) 貴州省農(nóng)業(yè)攻關(guān)項(xiàng)目(黔科合NY[2015]3014-1號)
【分類號】：S436.43

【參考文獻(xiàn)】

相關(guān)期刊論文 前2條

1 劉建業(yè);錢蕾;蔣興川;和淑琪;李正躍;桂富榮;;CO_2濃度升高對西花薊馬和花薊馬成蟲體內(nèi)解毒酶和保護(hù)酶活性的影響[J];昆蟲學(xué)報(bào);2014年07期

2 穆丹;付建玉;劉守安;韓寶瑜;;蟲害誘導(dǎo)的植物揮發(fā)物代謝調(diào)控機(jī)制研究進(jìn)展[J];生態(tài)學(xué)報(bào);2010年15期

相關(guān)博士學(xué)位論文 前1條

1 王永麗;斜紋夜蛾誘導(dǎo)大豆抗蟲防御反應(yīng)轉(zhuǎn)錄譜分析和分子功能研究[D];南京農(nóng)業(yè)大學(xué);2014年

【共引文獻(xiàn)】

相關(guān)期刊論文 前10條

1 劉建業(yè);錢蕾;可芮;陳曉燕;李正躍;桂富榮;;CO_2濃度升高對取食不同寄主的西花薊馬和花薊馬生理酶活性的影響[J];植物保護(hù)學(xué)報(bào);2017年01期

2 雷舒;李喜旺;孫曉玲;王志英;辛肇軍;;茶尺蠖為害提高鄰近茶苗對茶尺蠖幼蟲的防御能力[J];茶葉科學(xué);2016年06期

3 王瑩;孫麗麗;薩喜雅爾圖;王志英;;3種殺蟲劑對榆紫葉甲成蟲離體保護(hù)酶與解毒酶活性的影響[J];中國農(nóng)學(xué)通報(bào);2016年26期

4 陳曉燕;李永川;郝若詩;可芮;李亞紅;楊學(xué)存;桂富榮;;CO_2濃度升高對粘蟲幼蟲體內(nèi)酶活性的影響[J];應(yīng)用昆蟲學(xué)報(bào);2016年03期

5 來雨晴;王美仙;解瑩然;劉燕;;華山松冬季揮發(fā)物檢測分析[J];浙江農(nóng)業(yè)學(xué)報(bào);2016年02期

6 李寧;張世澤;劉同先;;二氧化碳濃度和溫度升高對煙粉虱主要保護(hù)酶和解毒酶活性的影響[J];植物保護(hù)學(xué)報(bào);2016年01期

7 阮楨媛;喬璐;白冰;;植物對它類生物應(yīng)答反應(yīng)的研究現(xiàn)狀及展望[J];環(huán)境昆蟲學(xué)報(bào);2016年05期

8 曹增;王金娜;張友軍;吳青君;謝文;王少麗;;煙粉虱取食感染TYLCV番茄對其解毒酶和保護(hù)酶活性的影響[J];植物保護(hù);2015年06期

9 趙正萍;嵇保中;劉曙雯;王麗平;許忠祥;;松梢隱翅蟲對松梢揮發(fā)物的選擇[J];環(huán)境昆蟲學(xué)報(bào);2015年05期

10 錢蕾;和淑琪;劉建業(yè);可芮;郝若詩;桂富榮;;在CO_2濃度升高條件下西花薊馬和花薊馬的生長發(fā)育及繁殖力比較[J];環(huán)境昆蟲學(xué)報(bào);2015年04期

【二級參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 蔣興川;李志華;曹志勇;和淑琪;李正躍;劉建業(yè);桂富榮;;蔬菜花期薊馬的種群動態(tài)與空間分布研究[J];應(yīng)用昆蟲學(xué)報(bào);2013年06期

2 史亮;李鴻波;金學(xué)柱;王建軍;杜予州;;低溫脅迫對西花薊馬抗氧化酶活性的影響[J];應(yīng)用昆蟲學(xué)報(bào);2013年04期

3 曾蕓蕓;黃文坤;蘇麗;吳剛;莊靜;趙萬云;華紅霞;李俊生;肖能文;熊燕飛;;高CO_2濃度對水稻褐飛虱體內(nèi)營養(yǎng)物質(zhì)及酶活性的影響[J];中國科學(xué):生命科學(xué);2012年09期

4 郭文娟;陸馳宇;熊應(yīng)強(qiáng);馬鶴娟;李紹勤;;轉(zhuǎn)cry1Ab/cry1Ac基因水稻對大螟幼蟲體內(nèi)三種保護(hù)酶活性的影響[J];昆蟲學(xué)報(bào);2012年08期

5 孟建玉;張長禹;雷朝亮;;紫外線脅迫對棉鈴蟲成蟲乙酰膽堿酯酶和羧酸酯酶的影響[J];貴州農(nóng)業(yè)科學(xué);2012年04期

6 劉玉坤;王渭霞;傅強(qiáng);賴鳳香;羅舉;;寄主植物對3種稻飛虱解毒酶和保護(hù)酶活性的影響[J];中國水稻科學(xué);2011年06期

7 呂要斌;張治軍;吳青君;杜予州;張宏瑞;于毅;王恩東;王鳴華;王滿V，

本文編號：2197973