【摘要】：目的探討長(zhǎng)鏈非編碼核醣核酸�；撬嵘险{(diào)基因1(lncRNA-TUG1)在肺腺癌組織中的表達(dá)及其對(duì)肺腺癌A549細(xì)胞生長(zhǎng)的影響。方法選取2012年1月-2014年12月間于天津市第一中心醫(yī)院胸外科行手術(shù)切除的肺腺癌患者的腫瘤組織及對(duì)應(yīng)癌旁組織40例。運(yùn)用實(shí)時(shí)熒光定量聚合酶鏈反應(yīng)(qRT-PCR)技術(shù)檢測(cè)lncRNA-TUG1在組織中的表達(dá)水平,分析lncRNA-TUG1表達(dá)與患者臨床病例資料間的相關(guān)性;通過(guò)si RNA沉默人肺腺癌A549細(xì)胞中l(wèi)ncRNA-TUG1的表達(dá),采用噻唑藍(lán)實(shí)驗(yàn)檢測(cè)沉默lncRNA-TUG1后A549細(xì)胞增殖變化,流式細(xì)胞儀檢測(cè)細(xì)胞凋亡變化,qRT-PCR及Western blot檢測(cè)P16表達(dá)變化。結(jié)果 lncRNA-TUG1在肺癌組織中表達(dá)水平高于對(duì)應(yīng)癌旁組織(t=3.873,P=0.000),肺癌組織中l(wèi)ncRNA-TUG1高表達(dá)與腫瘤直徑增大(P=0.033)及高TNM分期(P=0.045)相關(guān);lncRNA-TUG1特異性si RNA轉(zhuǎn)染A549細(xì)胞后可抑制細(xì)胞增殖(P=0.041)并上調(diào)凋亡細(xì)胞比例(t=3.206,P=0.007);qRT-PCR及Western blot結(jié)果證實(shí),沉默lncRNA-TUG1后可促進(jìn)P16 m RNA及蛋白表達(dá)水平(P=0.000)。結(jié)論 lncRNA-TUG1在肺腺癌組織中表達(dá)上調(diào)并與腫瘤惡性臨床病理特征有關(guān),lncRNA-TUG1可能通過(guò)下調(diào)抑癌基因P16的表達(dá)來(lái)促進(jìn)肺腺癌生長(zhǎng)。
[Abstract]:Objective to investigate the expression of long chain noncoding ribonucleic acid taurine up-regulated gene 1 (lncRNA-TUG1) in lung adenocarcinoma and its effect on the growth of lung adenocarcinoma cell line A549. Methods from January 2012 to December 2014, 40 patients with lung adenocarcinoma were selected from thoracic surgery department of Tianjin first Central Hospital. Real-time fluorescence quantitative polymerase chain reaction (qRT-PCR) was used to detect the expression of lncRNA-TUG1 in human lung adenocarcinoma A549 cells, and to analyze the correlation between the expression of lncRNA-TUG1 and the clinical data of patients, and to silence the expression of lncRNA-TUG1 in human lung adenocarcinoma A549 cells by si RNA. The proliferation of A549 cells after silencing lncRNA-TUG1 was detected by thiazolyl blue assay, apoptosis was detected by flow cytometry and P16 expression was detected by Western blot. Results the expression level of lncRNA-TUG1 in lung cancer tissues was higher than that in corresponding adjacent tissues (Tn3.873P0. 000). The high expression of lncRNA-TUG1 in lung cancer tissues was associated with tumor diameter enlargement (Pn0. 033) and high TNM stage (P0. 045). The expression of lncRNA-TUG1 in A549 cells was inhibited by transfection of LNcRNA-TUG1 specific si RNA (P0. 041). The results of QRT-PCR and Western blot showed that the proportion of apoptotic cells (tr 3.206) was adjusted by QRT-PCR and Western blot. Silencing lncRNA-TUG1 could promote the expression of P16 m RNA and protein (P0. 000). Conclusion the expression of lncRNA-TUG1 in lung adenocarcinoma is up-regulated and related to the malignant clinicopathological features of lung adenocarcinoma, which may promote the growth of lung adenocarcinoma by down-regulating the expression of tumor suppressor gene P16.
【作者單位】： 天津中醫(yī)藥大學(xué);天津市第一中心醫(yī)院免疫科;
【基金】：天津市衛(wèi)生局中醫(yī)處課題基金項(xiàng)目(No:2015047)
【分類號(hào)】：R734.2

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