本文選題：凡納濱對蝦 + 幼蝦�。� 參考：《上海海洋大學(xué)》2017年碩士論文

【摘要】：為探究凡納濱對蝦養(yǎng)殖2-3月齡階段的生長差異、生長相關(guān)基因及生長標(biāo)記,本實驗通過采集對蝦生長第28、38、48、58、68、73天的凡納濱對蝦,共取樣857只。按照生長速率不同將體重排名的前10%(14只)、中間10%(14只)、最后10%(14只)分為生長速率高中低三組。根據(jù)統(tǒng)計量用冪函數(shù)W=aLb進(jìn)行體長體重生長關(guān)系的擬合;體長體重取對數(shù),確定所有樣品在內(nèi)及不同生長速率組最佳直線方程;同時計算不同生長速率組對蝦的絕對日增長、絕對日增重和相對日增長率、相對日增重率,以探究不同生長速率組對蝦的生長差異。通過2-ΔΔCT方法對不同生長速率組不同生長時期的樣品進(jìn)行RPL18、SNRPG、RPL7基因相對表達(dá)量的分析。最后以28天的樣品對多態(tài)性較好的15對微衛(wèi)星引物進(jìn)行體重與位點相關(guān)性的分析。結(jié)果發(fā)現(xiàn):首先,相同生長時期的三組蝦生長速率顯著差異(P95%),將這些生長速率不同的蝦作為一個整體來分析蝦的生長情況可能會出現(xiàn)一定偏差。其次,體長與體重的回歸結(jié)果為:冪函數(shù)W所有樣品=1χ10-5L3.0051,直線方程y所有樣品=3x-4.92,不同生長速率組的直線方程分別為y高=3.41x-5.71,y中=3.21x-5.30,y低=2.92x-4.78;直線方程中斜率與生長速率呈正相關(guān),三組線性回歸方程能更加確切地反映出三組蝦的真實生長情況;同時發(fā)現(xiàn),不同生長速率蝦的絕對生長(絕對日增長和絕對日增重)符合對蝦的實際生長情況,而相對生長(相對日增長率和相對日增重率)與對蝦的實際生長情況相反,建議以后分析對蝦生長特性時參數(shù)選擇絕對生長值更為適宜。對于表達(dá)量的研究表明,不同生長速率組RPL18、SNRPG基因的相對表達(dá)量差異不顯著(P0.05),但除28天外,均表現(xiàn)為高速率組低速率組;RPL7基因相對表達(dá)量與體長的相關(guān)性高于體重,并且均表現(xiàn)為低速率組高中速率組,推測RPL7基因極大可能的參與凡納濱對蝦生長的負(fù)調(diào)控。本研究得出與體重存在相關(guān)性的TUMXLV 10.284微衛(wèi)星引物,結(jié)果表明BB基因型為高速率組和中速率組中的優(yōu)勢基因型(平均體重:3.39±0.37g、2.54±0.30 g),AC、CC為低速率組中的優(yōu)勢基因型(平均體重:0.53±0.10g、0.98±0.3g);所有樣品在內(nèi)的數(shù)據(jù)顯示BB、AB基因型對應(yīng)的體重(2.85±0.31 g、2.73±0.51g)最高,AC、CC基因型對應(yīng)的體重(1.18±1.12 g、1.45±0.36g)最低,且體重差異顯著(P0.05)。并且發(fā)現(xiàn)帶B基因型的個體體重均較高,帶C基因型的個體體重均較低,且差異顯著(P0.05)。
[Abstract]:In order to investigate the growth difference, growth related genes and growth markers of Penaeus vannamei cultured in 2-3 months of age, 857 prawns were collected from the 28 ~ (38) ~ (th) ~ (48) ~ (th) ~ (-) day of the growth of Penaeus vannamei (P.vannamei) in this experiment. According to the growth rate, the top 10% (14), the middle 10% (14) and the last 10% (14) were divided into three groups. According to the statistics, the relationship between body length and body weight was fitted by power function Wlb; the logarithm of body weight was taken to determine the best linear equation for all samples and groups of different growth rates; and the absolute daily growth of Penaeus penaeus at different growth rates was calculated. Absolute daily gain, relative daily growth rate and relative daily gain rate were used to explore the growth differences of shrimp in different growth rates. The relative expression of RPL18, SNRPGN RPL7 gene in different growth rate groups was analyzed by 2- 螖 CT. At last, 15 pairs of microsatellite primers with good polymorphism were used to analyze the correlation between body weight and locus. The results showed that: first, the growth rate of the three groups of shrimp in the same growth period was significantly different (P95%). Secondly, the regression results of body length and body weight were as follows: power function W all samples 蠂 ~ (-1) X ~ (-5) L ~ (3.0051), linear equation y _ y all samples ~ (3x-4.92), the linear equations of different growth rate groups were y ~ (3.41) -5.71 ~ (-1) y low 2.922x-4.78, and the slope of the linear equation was positively correlated with growth rate. Three groups of linear regression equations can more accurately reflect the actual growth of the three groups of shrimp, and found that the absolute growth (absolute daily growth and absolute daily gain) of shrimp at different growth rates is in line with the actual growth of Penaeus Monodon. However, the relative growth (relative daily growth rate and relative daily gain rate) is contrary to the actual growth of Penaeus Monodon. It is suggested that it is more appropriate to choose the absolute growth value when analyzing the growth characteristics of Penaeus penaeus in the future. The results showed that there was no significant difference in the relative expression of RPL18SN-SNRPG gene among different growth rate groups (P0.05), but the correlation between the relative expression of RPL7 gene and body length was higher than that of body length in high rate group and low rate group, except for 28 days. The RPL7 gene may be involved in the negative regulation of the growth of Penaeus vannamei. In this study, the microsatellite primers TUMXLV 10.284 correlated with body weight were obtained. The results showed that BB genotype was the dominant genotype in high rate group and middle rate group (mean weight: 3.39 鹵0.37 g / 2.54 鹵0.30 g). ACCC was the dominant genotype in low rate group (mean weight was 0.53 鹵0.10 g / 0.98 鹵0.3 g), and all samples showed that the weight of BBAB genotype corresponded to that of BBAB genotype. (2.85 鹵0.31 g / L 2.73 鹵0.51g) the highest weight corresponding to the ACCC genotype was the lowest (1.18 鹵1.12 g / L 1.45 鹵0.36 g). And the weight difference was significant (P0.05). It was found that the individuals with genotype B and genotype C had higher body weight and lower body weight than those with genotype C, and the difference was significant (P0.05).
【學(xué)位授予單位】：上海海洋大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：S968.22

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