本文選題：肌源性干細(xì)胞 + Wnt信號(hào)通路��； 參考：《時(shí)珍國醫(yī)國藥》2017年07期

【摘要】：目的 探討黃芪多糖干預(yù)的肌源性干細(xì)胞Wnt/β-catenin信號(hào)通路的相關(guān)基因表達(dá)的變化。方法 將經(jīng)混合酶消化法、多次差速貼壁法分離純化的MDSCs隨機(jī)分為6組,進(jìn)行實(shí)驗(yàn)干預(yù),即空白對(duì)照組、APS組、Wnt激動(dòng)劑組、APS+Wnt激動(dòng)劑組、Wnt抑制劑組及APS+Wnt抑制劑組。Realtime PCR檢測MDSCs Wnt信號(hào)通路上下游節(jié)點(diǎn)基因Wnt3、β-catenin及MDSCs增殖分化相關(guān)基因CyclinD1、c-myc、CD34mRNA的表達(dá)變化。結(jié)果 流式細(xì)胞儀檢測結(jié)果表明MDSCs CD34、Sca-1陽性。倒置顯微鏡下觀察,APS組、Wnt激動(dòng)劑組、APS+Wnt激動(dòng)劑組MDSCs較空白對(duì)照組生長密集,APS+Wnt激動(dòng)劑組聚團(tuán)明顯,Wnt抑制劑組MDSCs增殖明顯受到抑制。PCR檢測結(jié)果顯示:APS+激動(dòng)劑干預(yù)組的Wnt信號(hào)通路各節(jié)點(diǎn)基因表達(dá)水平最高,Wnt激動(dòng)劑組與APS組其次,空白對(duì)照組最低。結(jié)論 在黃芪多糖的干預(yù)作用下,持續(xù)激活Wnt信號(hào)通路能夠促進(jìn)MDSCs的增殖分化。
[Abstract]:Objective to investigate the expression of Wnt / 尾-catenin signaling pathway in myogenic stem cells treated with astragalus polysaccharide. Methods MDSCs isolated and purified by mixed enzyme digestion and multiple differential adherence were randomly divided into 6 groups for experimental intervention. Wnt agonist group and APS Wnt inhibitor group. Realtime PCR was used to detect the expression of Wnt gene Wnt3, 尾 -catenin and CyclinD1c-mycCD34 mRNA in MDSCs Wnt signaling pathway. Results the results of flow cytometry showed that MDSCs CD34 and Sca-1 were positive. Observation of MDSCs in APS Wnt agonist group under inverted microscope compared with APS Wnt agonist group in blank control group the proliferation of MDSCs in APS Wnt agonist group was significantly inhibited. PCR results showed that the proliferation of MDSCs was significantly inhibited in the control group treated with APS Wnt agonist. PCR results showed that the MDSCs were significantly inhibited in the control group treated with the APS agonist. The highest level of gene expression in each node of Wnt signaling pathway was found in Wnt agonist group and APS group. Blank control group was the lowest. Conclusion the continuous activation of Wnt signaling pathway can promote the proliferation and differentiation of MDSCs under the intervention of astragalus polysaccharide.
【作者單位】： 天津中醫(yī)藥大學(xué);
【分類號(hào)】：R285

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