本文選題：鴨 + STING基因��； 參考：《畜牧與獸醫(yī)》2017年07期

【摘要】：根據(jù)鴨STING基因預(yù)測(cè)序列,設(shè)計(jì)了2對(duì)特異性引物,通過試驗(yàn)條件摸索,首次建立了基于SYBR Green熒光定量PCR檢測(cè)鴨STING基因方法,并使用該方法對(duì)STING在鴨不同組織中的含量進(jìn)行檢測(cè)。結(jié)果:本研究建立的檢測(cè)方法,能檢測(cè)低至4.8×10~3拷貝數(shù)/μL的樣品;其標(biāo)準(zhǔn)曲線線性范圍是3.5×10~(-5)~0.27 ng/μL,具有良好的重復(fù)性。組織分布研究發(fā)現(xiàn),STING在腺胃中表達(dá)量最高,在氣管、肺臟和小腸中表達(dá)量較高,在脾、腎、法氏囊、肌胃、肝、盲腸呈中等表達(dá),在肌肉和皮膚中表達(dá)量最低。本研究成功建立了鴨STING基因的熒光定量PCR檢測(cè)方法,為深入研究STING基因在鴨抗病毒感染中的作用奠定了基礎(chǔ)。
[Abstract]:According to the prediction sequence of duck STING gene, 2 pairs of specific primers were designed. The method of detecting duck STING gene based on SYBR Green fluorescence quantitative PCR was established for the first time. The method was used to detect the content of STING in different tissues of ducks. Results: the detection method established in this study can detect a copy of 4.8 x 10~3 copies. The linear range of the standard curve is 3.5 * 10~ (-5) ~0.27 ng/ mu L and has good repeatability. The tissue distribution study found that the expression of STING in the stomach is the highest, the expression in the trachea, the lungs and the small intestine is high, and the expression in the spleen, kidney, bursa of the bursa, the stomach, the liver and the cecum is the lowest. This study is the lowest in the muscles and the skin. The fluorescence quantitative PCR detection method of duck STING gene was successfully established, which laid a foundation for further research on the role of STING gene in duck virus infection.
【作者單位】： 上海交通大學(xué)農(nóng)業(yè)與生物學(xué)院/上海市獸醫(yī)生物技術(shù)重點(diǎn)實(shí)驗(yàn)室;上海市寶山區(qū)動(dòng)物疫病預(yù)防控制中心;
【基金】：國(guó)家自然科學(xué)基金(31672524)
【分類號(hào)】：S834
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本文編號(hào)：2107872