發(fā)布時(shí)間：2018-07-02 12:13

  本文選題：豬A組輪狀病毒 + VP�。� 參考：《中國預(yù)防獸醫(yī)學(xué)報(bào)》2017年04期

【摘要】：為研究豬A組輪狀病毒(PRV-A)血凝蛋白(VP4)、群抗原蛋白(VP6)和中和抗原蛋白(VP7)編碼基因疫苗聯(lián)合免疫的效力,本研究將表達(dá)PRV-AVP4、VP6、VP7蛋白基因的真核表達(dá)質(zhì)粒pVAX1-VP4、pVAX1-VP6、pVAX1-VP7以pVAX1-VP4+pVAX1-VP7(B組)和pVAX1-VP4+p VAX1-VP6+pVAX1-VP7(A組)設(shè)計(jì)試驗(yàn)組,分別以100μg/只經(jīng)肌肉注射途徑免疫BLAL/c小鼠,間隔2周以相同劑量、途徑加強(qiáng)免疫,共免疫3次,免疫后不同時(shí)間采血,采用ELISA檢測血清抗體。結(jié)果顯示,B組在免疫后14 d和42 d檢測到RV抗體陽性(P/N≥2),A組在免疫后14 d、28 d、42 d均檢測到RV抗體陽性(P/N≥2);脾臟免疫相關(guān)細(xì)胞測定結(jié)果表明,A組的CD4~+/CD8~+T淋巴細(xì)胞比值和CD19~+B細(xì)胞數(shù)量均高于B組,兩組與對(duì)照組相比差異顯著(p0.05);淋巴細(xì)胞轉(zhuǎn)化試驗(yàn)顯示A組的SI值均高于B組,并且42 d時(shí)A、B兩組差異顯著(p0.05)。上述結(jié)果表明,3組分聯(lián)合免疫的DNA疫苗可以誘導(dǎo)小鼠產(chǎn)生較強(qiáng)的體液和細(xì)胞免疫應(yīng)答,為PRV-A的核酸疫苗研究奠定了基礎(chǔ)。
[Abstract]:To study the efficacy of combined immunization of porcine group A rotavirus (PRV-A) hemagglutinin (VP4), group antigen protein (VP6) and neutralizing antigen protein (VP7). In this study, the eukaryotic expression plasmid pVAX1-VP4pVAX1-VP6 pVAX1-VP7 (group B) and pVAX1-VP4pVAX1-VP7 (group A) were designed to express PRV-AVP4VP6pVAX1-VP7 (group A), and BLALP-c mice were immunized by intramuscular injection of 100 渭 g / a of pVAX1-VP4pVAX1-VP7 (group A). The blood samples were collected at different time after immunization and the serum antibodies were detected by Elisa. The results showed that RV antibody positive (P / N 鈮，

本文編號(hào)：2090084