本文選題：水稻 + T-DNA插入�。� 參考：《中國生物防治學報》2017年01期

【摘要】：本文評價了T-DNA插入對水稻農(nóng)藝性狀與抗蟲性、褐飛虱Nilaparvata lugens St?l取食選擇性及3種刺吸式口器害蟲種群密度的影響,以分析轉基因操作及外源基因插入可能引起的非預期效應。結果表明,供試ZD1640-3、ZD1640-19、ZD1640-23和ZD1640-24共4個突變體株系T-DNA插入位點附近基因的表達均不受影響;除個別株系的株高、每穗粒數(shù)或結實率有降低外,主要農(nóng)藝性狀參數(shù)與受體親本無顯著差異。除ZD1640-24外,突變體各株系上褐飛虱、白背飛虱Sogatella furcifera(Horváth)和黑尾葉蟬Nephotettix bipunctatus(Fabricius)平均種群密度與受體親本無顯著差異;且4個突變體株系上3種害蟲種群密度在大多數(shù)情況下與受體親本也無顯著差異。與受體親本相比,突變體株系ZD1640-24對褐飛虱的抗性及取食選擇性無顯著影響。綜合分析認為,T-DNA基因間插入并沒有引起水稻發(fā)生顯著的非預期變化,個別突變體某些考查指標的變化主要來自于個體差異。
[Abstract]:The effects of T-DNA insertion on agronomic traits and insect resistance of rice, feeding selectivity of Nilaparvata lugens Stl and population density of three kinds of sucking insect pests were evaluated in order to analyze the unexpected effects caused by transgenic operation and exogenous gene insertion. The results showed that the expression of genes near the T-DNA insertion site of ZD1640-3 and ZD1640-24 mutant lines were not affected, except for the plant height, grain number per panicle or seed setting rate of individual lines, there was no significant difference between the main agronomic characters and the recipient parents. Except for ZD1640-24, there was no significant difference between the average population density of BPH, Sogatella furcifera (Horv 謾 th) and Nephotettix bipunctatus (Fabricius). The population densities of the three pests in the four mutants were not significantly different from those of the recipient parents in most cases. Compared with the recipient parents, the mutant ZD1640-24 had no significant effect on the resistance and feeding selectivity of brown planthopper. The comprehensive analysis showed that the insertion of T-DNA gene did not cause significant unexpected changes in rice, and the changes of some indexes in individual mutants were mainly due to individual differences.
【作者單位】： 浙江大學昆蟲科學研究所/水稻生物學重點實驗室/農(nóng)業(yè)部農(nóng)業(yè)昆蟲學重點實驗室;
【基金】：國家轉基因生物新品種培育重大專項(2014ZX08011-001)
【分類號】：S511;S435.11

【參考文獻】

相關期刊論文 前3條

1 SHI Zhao-peng;DU Shang-gen;YANG Guo-qing;LU Zhen-zhen;WU Jin-cai;;Effects of Pesticide Applications on the Biochemical Properties of Transgenic cry 2A Rice and the Life History Parameters of Nilaparvata lugens Stl (Homptera: Delphacidae)[J];Journal of Integrative Agriculture;2013年09期

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本文編號：2060681