本文選題：蓮草直胸跳甲 + 小熱激蛋白　； 參考：《環(huán)境昆蟲學(xué)報(bào)》2017年04期

【摘要】：sHsp21(小熱激蛋白)是小熱激蛋白家族的成員,在外界環(huán)境壓力下能夠被誘導(dǎo),熱激后能夠調(diào)節(jié)蛋白質(zhì)的折疊和保護(hù)細(xì)胞免受外界脅迫。本文研究了入侵雜草空心蓮子草Alternanthera philoxeroides生防天敵蓮草直胸跳甲的一個(gè)關(guān)鍵基因sHsp21,并對(duì)其進(jìn)行熱激誘導(dǎo)表達(dá)以及RACE全長(zhǎng)擴(kuò)增。獲得全長(zhǎng)cDNA 693 bp并完整的開(kāi)放閱讀框567 bp,共編碼188個(gè)氨基酸。以30℃為對(duì)照,分別測(cè)定蓮草直胸跳甲Agasicles hygrophila SelmanVogt長(zhǎng)沙種群和海南種群在模擬夏季特定10∶00-14∶00時(shí)段36℃、39℃高溫條件下sHsp21的表達(dá)量。連續(xù)熱激6 d,每天4 h熱激后取樣,實(shí)時(shí)熒光定量PCR分析顯示:湖南種群高溫39℃和36℃誘導(dǎo)下sHsp21表達(dá)量都顯著高于對(duì)照組30℃;海南種群36℃誘導(dǎo)下的表達(dá)量與對(duì)照組30℃差異不顯著,39℃誘導(dǎo)下的表達(dá)量顯著高于對(duì)照組30℃和36℃。結(jié)果表明,sHsp21在湖南和海南兩地理種群不同耐熱能力上起到重要作用,并且sHsp21的高表達(dá)可能對(duì)其蟲體產(chǎn)生副作用,為下一步深入探究其生物學(xué)功能奠定了一定的基礎(chǔ)。
[Abstract]:SHsp21 (small heat shock protein) is a member of small heat shock protein family, which can be induced under environmental pressure. Heat shock can regulate protein folding and protect cells from external stress. In this paper, a key gene, sHsp21, which is a key gene for the biocontrol of Alternanthera philoxeroides, a natural enemy, was studied, and its expression was induced by heat shock and the full-length amplification of race was carried out. A full length cDNA of 693 BP and a complete open reading frame of 567 BP encoding 188 amino acids were obtained. Using 30 鈩，

本文編號(hào)：2055040