發(fā)布時間：2018-06-18 23:11

  本文選題：茶樹 + TS��； 參考：《茶葉科學》2017年03期

【摘要】：茶氨酸合成酶(Theanine synthetase,TS)基因是茶樹茶氨酸代謝過程中的關鍵酶基因。本研究以氨基酸含量差異明顯的親本及其雜交所得F_1子代為研究材料,克隆TS基因的c DNA序列,挖掘其SNPs位點,并成功將雜合SNP位點定位在遺傳連鎖群上。研究結果顯示,通過序列比對在親本間檢測到3個SNPs,驗證得到1個雜合的位點SNP735。將此位點成功轉化為dCAPS標記,該標記在子代中的基因型分離比為1∶1,利用該群體已構建的茶樹遺傳圖譜進行遺傳定位,將dCAPS標記定位在連鎖群LG03上,相鄰標記為TM299和TM517。聯(lián)合此標記及其相鄰標記與游離氨基酸總含量和茶氨酸含量進行統(tǒng)計分析,表明具有顯著的相關性。
[Abstract]:Theanine synthetase (Theanine synthetase) gene is a key enzyme gene in theanine metabolism of tea plants. In this study, the parents with obvious difference in amino acid content and their F _ 1 progenies were used as the materials to clone the cDNA sequence of TS gene, to excavate its SNPs locus, and to successfully locate the heterozygous SNP loci in the genetic linkage population. The results showed that three SNPs were detected between parents by sequence alignment, and a heterozygous locus SNP735 was identified. The locus was successfully transformed into a dCAPS marker with a genotypic segregation ratio of 1: 1 in the progeny. Using the constructed genetic map of tea tree in this population, the dCAPS marker was mapped to the linkage group LG03, and the adjacent markers were TM299 and TM517. Combined with this marker and its adjacent markers, the total content of free amino acids and theanine content were statistically analyzed.
【作者單位】： 中國農業(yè)科學院茶葉研究所國家茶樹改良中心;
【基金】：國家茶葉產業(yè)技術體系項目(CARS-023) 國家自然科學基金(31170624、31500568)
【分類號】：S571.1

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本文編號：2037200