本文選題：陸地棉 + 高分辨率熔解曲線�。� 參考：《棉花學(xué)報》2017年01期

【摘要】：【目的】定位徐州142無絮(XZ142w)突變體的短絨控制基因n2。【方法】以陸地棉(Gossypium hirsutum L.)徐州142(XZ142)×XZ142w的F2群體為研究對象,利用108個簡單重復(fù)序列(Simple sequence repeat,SSR)標(biāo)記對n2進(jìn)行初步定位,再根據(jù)2個親本材料中有單核苷酸多態(tài)性(Single nucleotide polymorphic,SNP)的差異基因設(shè)計50對SNP引物,用高分辨率熔解曲線(High resolution melting,HRM)技術(shù)從中篩選在親本間有多態(tài)性的SNP引物,并用于后代的基因分型。【結(jié)果】利用108個SSR標(biāo)記將n2初步定位在26號染色體的20.2c M的遺傳區(qū)間內(nèi);用HRM技術(shù)篩選到9對親本間有多態(tài)性的SNP引物,成功實現(xiàn)基因分型;并結(jié)合以SSR構(gòu)建的連鎖圖譜,將n2的遺傳區(qū)間縮小為19.5 c M,n2與最近的SNP標(biāo)記Cricaas20158遺傳距離為5.5 c M,且遺傳圖譜上的標(biāo)記與四倍體陸地棉測序物理圖譜基本一致。【結(jié)論】HRM技術(shù)可用于棉花中的SNP檢測和n2基因的定位。
[Abstract]:[objective] to localize the short velvet control gene n22.Methods to localize the XZ142w mutant of Xuzhou 142, Gossypium hirsutum L. The F2 population of Xuzhou 142XZ142w 脳 XZ142w was preliminarily located by 108 simple sequence repeats markers, and 50 pairs of SNPs were designed according to the single nucleotide polymorphisms single nucleotide polymorphism (SNPs) in two parents. High resolution fusion curve (HRM) technique was used to screen polymorphic primers from parents and to be used for genotyping of progeny. [results] using 108 SSR markers, N2 was preliminarily located in the genetic interval of 20.2cm on chromosome 26. Nine pairs of SNP primers with polymorphism between parents were screened by HRM technique, and the genotyping was successfully achieved, and the linkage map constructed by SSR was used. The genetic interval of n2 was reduced to 19.5c Mn2 and the genetic distance of Cricaas20158, a recent SNP marker, was 5.5 cm, and the markers on the genetic map were basically consistent with the physical map of tetraploid upland cotton sequencing. [conclusion] HRM technique can be used in cotton. Detection of SNP and mapping of N2 gene.
【作者單位】： 中國農(nóng)業(yè)科學(xué)院棉花研究所/棉花生物學(xué)國家重點實驗室;
【基金】：國家863計劃(2013AA102601-01-14) 國家科技支撐計劃(2014BAD03B01)
【分類號】：S562

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