本文選題：牦牛 + FKBP��； 參考：《畜牧獸醫(yī)學(xué)報(bào)》2017年01期

【摘要】：本研究旨在了解牦牛FKBP6基因5′調(diào)控區(qū)和啟動(dòng)子區(qū)特征,為探討牦牛和犏牛睪丸組織中FKBP6基因差異表達(dá)機(jī)制提供依據(jù)。利用克隆測序獲得牦牛FKBP6基因5′調(diào)控區(qū)序列,采用生物信息學(xué)方法分析其序列特征;采用雙熒光素酶報(bào)告基因系統(tǒng)鑒定牦牛FKBP6基因核心啟動(dòng)子區(qū),利用生物信息學(xué)軟件預(yù)測與精子發(fā)生有關(guān)的轉(zhuǎn)錄因子結(jié)合位點(diǎn)。通過克隆測序和序列拼接獲得了1 354bp的牦牛FKBP6基因5′調(diào)控區(qū)序列,與普通牛的一致性為99.71%;牦牛FKBP6基因5′調(diào)控區(qū)序列含有潛在的啟動(dòng)子區(qū)、典型的CAAT-Box和CpG島,但未見TATA-Box;熒光素酶活性分析發(fā)現(xiàn),牦牛FKBP6基因的核心啟動(dòng)子區(qū)位于5′調(diào)控區(qū)的-263~-167nt區(qū)域,含有CAAT-Box、E-Box、CTCF和CREB等與精子發(fā)生相關(guān)的轉(zhuǎn)錄因子結(jié)合位點(diǎn)。牦牛FKBP6基因核心啟動(dòng)子的鑒定和精子發(fā)生相關(guān)轉(zhuǎn)錄因子結(jié)合位點(diǎn)的發(fā)現(xiàn)為進(jìn)一步研究牦牛睪丸組織中FKBP6基因的表達(dá)調(diào)控奠定了基礎(chǔ)。
[Abstract]:The aim of this study was to understand the characteristics of the 5 'regulatory region and promoter region of FKBP6 gene in yak and to provide evidence for the differential expression mechanism of FKBP6 gene in testis. The sequence of 5 'regulatory region of FKBP6 gene was obtained by cloning and sequencing, and the sequence characteristics of FKBP6 gene were analyzed by bioinformatics, and the core promoter region of FKBP6 gene was identified by double luciferase reporter gene system. Bioinformatics software was used to predict transcription factor binding sites related to spermatogenesis. The sequence of 5 '-regulatory region of 1 354bp FKBP6 gene was obtained by cloning and sequencing, and the sequence was 99.71, and the sequence of 5' -regulatory region of FKBP6 gene of yak contained potential promoter region, typical CAAT-Box and CpG island, the sequence of 5 '-regulatory region of FKBP6 gene of yak was found to be 99.71, and the sequence of 5' regulatory region of FKBP6 gene of yak contained potential promoter region. However, TATA-Box. luciferase activity analysis showed that the core promoter of FKBP6 gene was located in -263 ~ 167nt region of 5'regulatory region, which contained CAAT-BoxCF-BoxP6 CTCF and CREB transcription factor binding sites related to spermatogenesis. The identification of the core promoter of the FKBP6 gene and the discovery of spermatogenetic transcription factor binding sites in yak laid a foundation for further study on the expression and regulation of FKBP6 gene in yak testis.
【作者單位】： 南京農(nóng)業(yè)大學(xué)動(dòng)物科技學(xué)院;
【基金】：國家自然科學(xué)基金(30500360)
【分類號(hào)】：S823.85

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