本文選題：普通小麥煙農(nóng) + 幼苗��； 參考：《作物雜志》2016年04期

【摘要】：非生物逆境(高溫、低溫、干旱、鹽脅迫等)嚴(yán)重影響作物生長,研究參與逆境脅迫應(yīng)答基因具有重要的理論意義和應(yīng)用價(jià)值。超氧化物歧化酶(superoxide dismutase,SOD)是一種廣泛存在于真核生物中的金屬酶類,在植物的抗逆性中起到重要作用。采用q RT-PCR技術(shù),分析普通小麥煙農(nóng)19幼苗葉中Fe SOD基因在鹽、脫落酸(ABA)、干旱、高溫、低溫脅迫過程中的的表達(dá)模式。結(jié)果表明:在逆境脅迫下,普通小麥煙農(nóng)19幼苗Fe SOD基因的表達(dá)量大體呈現(xiàn)先上升后下降的趨勢。在37℃高溫、-4℃低溫、300mmol/L Na Cl、30%的PEG-6000和100μmol/L ABA脅迫下,Fe SOD基因的表達(dá)量分別在3、3、6、48和24h時(shí)最高,分別為對照的34.0、4.6、4.3、5.8、13.5和3.3倍,差異均達(dá)到顯著水平,說明Fe SOD基因在普通小麥煙農(nóng)19幼苗逆境脅迫中發(fā)揮著重要的調(diào)控功能,為進(jìn)一步了解小麥抗逆分子機(jī)制和改良小麥品種提供理論依據(jù)。
[Abstract]:Abiotic stresses (high temperature, low temperature, drought, salt stress, etc.) seriously affect crop growth. It is of great theoretical significance and practical value to study the genes involved in stress response. Superoxide dismutase (SOD) is a kind of metalloenzymes widely found in eukaryotes, which plays an important role in plant stress resistance. The expression patterns of Fe SOD gene in leaves of common wheat Yannong 19 seedlings were analyzed by using qRT-PCR during salt, abscisic acid, drought, high temperature and low temperature stress. The results showed that the expression of Fe SOD gene in common wheat Yannong 19 seedlings increased firstly and then decreased under stress. Under the stress of 30% PEG-6000 and 100 渭 mol / L ABA at 37 鈩，

本文編號(hào)：2015237