本文選題：生物信息學(xué) + 甘藍(lán)型油菜　； 參考：《植物學(xué)報(bào)》2016年05期

【摘要】：在高等植物花藥發(fā)育和花粉形成中,MYB轉(zhuǎn)錄因子起著非常重要的作用,其中MYB80是參與絨氈層發(fā)育及引起雄性不育的重要轉(zhuǎn)錄因子。該研究以擬南芥(Arabidopsis thaliana)At MYB80為參考序列,通過(guò)BLAST比對(duì)分析,在白菜(Brassica rapa)、甘藍(lán)(B.oleracea)和甘藍(lán)型油菜(B.napus)中分別獲得MYB80基因的2、2和6個(gè)同源序列,運(yùn)用生物信息學(xué)方法對(duì)其核苷酸序列及編碼的氨基酸序列進(jìn)行組成成分、亞細(xì)胞定位、磷酸化位點(diǎn)、疏水性/親水性、蛋白質(zhì)二級(jí)、三級(jí)結(jié)構(gòu)和功能域分析。結(jié)果表明,MYB80轉(zhuǎn)錄因子亞細(xì)胞定位于細(xì)胞核,具有多個(gè)不同的磷酸化位點(diǎn),肽鏈表現(xiàn)為親水性;二級(jí)、三級(jí)結(jié)構(gòu)預(yù)測(cè)顯示,MYB80蛋白以α-螺旋和無(wú)規(guī)則卷曲為主要結(jié)構(gòu)元件;保守結(jié)構(gòu)域分析表明,其N端具有2個(gè)串聯(lián)的SANT功能域,屬于R2R3型MYB轉(zhuǎn)錄因子。多重序列比對(duì)和進(jìn)化樹(shù)分析結(jié)果表明,甘藍(lán)型油菜與白菜、甘藍(lán)的序列相似性大于92%,且MYB80轉(zhuǎn)錄因子的功能結(jié)構(gòu)域具有較高的同源性和較強(qiáng)的序列保守性。該研究結(jié)果對(duì)深入解析甘藍(lán)型油菜MYB80的生物學(xué)功能及育性調(diào)控的分子機(jī)理具有重要意義,為甘藍(lán)型油菜雜種優(yōu)勢(shì)利用提供了依據(jù)。
[Abstract]:MYB transcription factors play a very important role in anther development and pollen formation of higher plants, among which MYB80 is an important transcription factor involved in tapetum development and male sterility. Using Arabidopsis thaliana)At MYB80 as the reference sequence and blast comparison analysis, two and six homologous sequences of MYB80 gene were obtained in Brassica rapaena, B. oleracea and Brassica napus, respectively. The composition, subcellular localization, phosphorylation site, hydrophobic / hydrophilic, protein secondary, tertiary structure and functional domain were analyzed by bioinformatics. The results showed that the MYB80 transcription factor subcells were located in the nucleus and had many different phosphorylation sites, and the peptide chain was hydrophilic, the second and third order structure prediction showed that the main structural elements of MYB80 protein were 偽 -helix and irregular curl. The analysis of conserved domain showed that the N-terminal had two sant domains, which belonged to R2R3 MYB transcription factor. The results of multiple sequence alignment and phylogenetic tree analysis showed that the sequence similarity between Brassica napus and Brassica campestris was higher than that in Brassica napus, and the functional domain of MYB80 transcription factor had high homology and strong conserved sequence. The results of this study are of great significance in understanding the biological function of MYB80 and the molecular mechanism of fertility regulation in Brassica napus, and provide a basis for the utilization of heterosis in Brassica napus L.
【作者單位】： 西南大學(xué)農(nóng)學(xué)與生物科技學(xué)院;
【基金】：國(guó)家重點(diǎn)研發(fā)計(jì)劃“七大農(nóng)作物育種”專項(xiàng)(No.2016YFD0101300) 國(guó)家自然科學(xué)基金(No.U1302266)
【分類號(hào)】：S565.4
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本文編號(hào)：2009772