本文選題：白木香 + 人工誘導��； 參考：《時珍國醫(yī)國藥》2017年05期

【摘要】：目的研究不同人工誘導方法對白木香結香過程中萜類相關基因表達量的影響。方法 "小孔滴注法"進行甲酸、甲酸結合鐮刀菌人工誘導試驗,手工割取誘導前、后12個月內(nèi)的白木香結香部位,實時熒光定量PCR法(qRT-PCR)測定As-HMGR、As-DXS1、As-DXS2基因表達水平。結果 As-HMGR、As-DXS1、As-DXS2回歸方程分別為Y=-3.580X+40.11、Y=-3.424X+38.38、Y=-3.728X+41.84,相關系數(shù)均大于0.997,線性關系均良好。甲酸、甲酸結合鐮刀菌誘導過程中,As-DXS1與As-DXS2的表達模式相近,前8個月表達水平較低,隨后顯著升高。甲酸誘導時,As-HMGR基因從2個月開始表達,呈現(xiàn)先升高后下降再升高的趨勢;甲酸結合鐮刀菌誘導時,初期相對表達量緩慢升高,6個月時達到峰值,隨后急劇下降。人工誘導12個月As-HMGR、As-DXS1的相對表達量,甲酸誘導高于甲酸結合鐮刀菌誘導(P0.05)。結論甲酸、甲酸結合鐮刀菌誘導白木香結香過程中As-HMGR基因在傷害早期響應傷害脅迫,As-DXS1、As-DXS2基因在后期響應,為進一步研究白木香萜類次生代謝產(chǎn)物表達與調(diào)控,改善人工沉香品質(zhì)奠定基礎。
[Abstract]:Objective to study the effect of different artificial induction methods on the expression of terpene related genes in the process of aroma formation. Methods Formic acid, formic acid combined with Fusarium mongolicum were used for formic acid, formic acid and Fusarium oxysporum to detect the expression level of As-DXS1As-DXS2 gene in As-HMGRN As-DXS1As-DXS2 gene by real-time fluorescence quantitative PCR within 12 months before and 12 months after induction. Results the regression equations of As-HMGRN As-DXS1 and As-DXS2 were Yan-3.580X 40.11X 40.11X 38.324X 38.324X 38.328X 41.84, and the correlation coefficients were all greater than 0.997.The linear relationship was good. The expression patterns of As-DXS1 and As-DXS2 were similar to those of As-DXS2 during the induction of formic acid and formic acid-bound Fusarium. The expression level of As-DXS1 was lower in the first eight months and then increased significantly. The expression of As-HMGR gene began from 2 months after formic acid induction, which increased first and then decreased and then increased. When formic acid was induced by Fusarium oxysporum, the relative expression level increased slowly, reached its peak at 6 months, and then decreased sharply. The relative expression of As-HMGRN As-DXS1 in artificial 12 months was higher than that induced by Fusarium formate. Conclusion in the process of formic acid, formic acid combined with Fusarium, As-HMGR gene responds to injury stress in the early stage of injury. In order to further study the expression and regulation of terpenoid secondary metabolites, As-DXS1 and As-DXS2 genes are in late response to injury stress. Improve the quality of artificial incense to lay the foundation.
【作者單位】： 廣東藥科大學藥學院;仙樂健康科技股份有限公司;廣東省微生物研究所省部共建華南應用微生物國家重點實驗室廣東省菌種保藏與應用重點實驗室廣東省微生物應用新技術公共實驗室;
【基金】：國家自然科學基金(No.81102418) 廣東省基礎與應用基礎研究專項資金(No.2014A030313583) 廣東省中醫(yī)藥局科研課題(No.20161151) 廣東省科學院野外科學實驗站基金項目(No.Sytz201504;No.Sytz201511)
【分類號】：S567.19

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