本文選題：巨尾桉 + 銅分子伴侶　； 參考：《華僑大學(xué)學(xué)報(自然科學(xué)版)》2017年03期

【摘要】：以巨尾桉為研究對象,克隆得到巨尾桉銅分子伴侶(CCS)基因(GenBank注冊號為KJ755351.1),生物信息學(xué)分析表明:EuCCS蛋白定位于葉綠體,與龍眼同源基因的一致性達到99%.巨尾桉CCS的原核表達載體pET-EuCCS在大腸桿菌細(xì)胞內(nèi)可以穩(wěn)定表達,超氧化物歧化酶(SOD)活檢測結(jié)果表明:轉(zhuǎn)化菌株的SOD酶活性比對照菌株高,說明外源基因EuCCS具有生物學(xué)活性.利用熒光定量聚合酶鏈?zhǔn)椒磻?yīng)(PCR)技術(shù)分析CCS在巨尾桉中的表達特性,結(jié)果表明:在植株生長旺盛的部位,CCS表達量較大,如植株幼葉,隨植株葉片慢慢衰老,CCS的表達量也隨之下降;在組培苗中,葉片的CCS表達量最大,其次是莖.
[Abstract]:Taking Eucalyptus grandis as the research object, the GenBank registration number of CCS gene was KJ755351.1. Bioinformatics analysis showed that the protein was located in chloroplast and the homologous gene was 99g. The prokaryotic expression vector pET-EuCCS of Eucalyptus grandis CCS was stably expressed in Escherichia coli cells, and the activity of superoxide dismutase (SOD) was detected. The results showed that the SOD activity of the transformed strain was higher than that of the control strain, indicating that the exogenous gene EuCCS had biological activity. Fluorescence quantitative polymerase chain reaction (PCR) technique was used to analyze the expression characteristics of CCS in Eucalyptus grandis. The expression of CCS decreased with the senescence of plant leaves, and the CCS expression was the highest in the plantlets in tissue culture, followed by the stems.
【作者單位】： 華僑大學(xué)化工學(xué)院;
【基金】：國家自然科學(xué)基金資助項目(31300497)
【分類號】：Q943.2;S792.39

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