本文選題：綿羊 + FSHR基因　； 參考：《江蘇農(nóng)業(yè)學報》2017年03期

【摘要】：根據(jù)Gen Bank中綿羊促卵泡激素受體基因(FSHR)的mRNA序列(登錄號:NM_001009289.1)設計引物,反轉錄PCR(RT-PCR)擴增蛋白編碼區(qū)(CDs)區(qū),以獲取綿羊FSHR基因可變剪接體,并通過Western blot驗證其在組織中的表達,通過免疫組織化學方法檢測其在卵泡中的表達位置。結果表明,在綿羊卵巢中除了全長的、編碼695個氨基酸的FSHR表達產(chǎn)物外,還鑒定到分別編碼694個、633個、595個、582個和533個氨基酸的FSHR可變剪接形式的轉錄產(chǎn)物。在卵泡中主要以695(694)、633和595 3種轉錄本形式存在。但在顆粒細胞中綿羊促卵泡激素受體主要以經(jīng)典分子即695個氨基酸的形式存在。在卵泡發(fā)育過程中,FSHR蛋白質含量發(fā)生變化:在初級卵泡中,FSHR主要在卵母細胞中表達,而不是在單層的顆粒細胞中表達,在次級卵泡中,FSHR在顆粒細胞和卵母細胞中均不表達,到三級卵泡(有腔卵泡)時,FSHR蛋白質在顆粒細胞中表達豐富�？梢�,綿羊FSHR基因雖然存在可變剪接體的結構,但其功能主要取決于經(jīng)典型的695個氨基酸蛋白質分子,并且對初級卵泡的發(fā)育具有一定的作用。
[Abstract]:According to the mRNA sequence of sheep follicle stimulating hormone receptor gene (FSHR) in Gen Bank (accession number: NMash009289.1), primers were designed to amplify the protein coding region (CDss) by reverse transcription PCRRT-PCR (reverse transcription PCRRT-PCR) to obtain the variable splicing of sheep FSHR gene, and its expression in tissues was verified by Western blot. Immunohistochemical method was used to detect its expression in follicles. The results showed that in addition to the full-length FSHR expression products encoding 695 amino acids in ovaries of sheep, the mutable splicing transcripts of FSHR were also identified. In follicles, there are three transcription forms: 695, 694, 633 and 595, respectively. However, sheep follicle stimulating hormone receptor in granulosa cells mainly exists in the form of 695 amino acids. The protein content of FSHR changed during follicular development: in primary follicles, FSHR was expressed mainly in oocytes, not in monolayer granulosa cells, but in secondary follicles, FSHR was not expressed in granulosa cells and oocytes. FSHR protein was abundant in granulosa cells by the third stage follicle (with antral follicles). It can be seen that although sheep FSHR gene has a variable splice structure, its function mainly depends on the classical 695 amino acid protein molecules and plays a certain role in the development of primary follicles.
【作者單位】： 新疆畜牧科學院生物技術研究所;
【基金】：新疆維吾爾自治區(qū)自然科學基金項目(2014211A068)
【分類號】：S826

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