本文選題：代謝綜合征 + 骨形態(tài)發(fā)生蛋白-7�。� 參考：《山西醫(yī)科大學(xué)》2017年碩士論文

【摘要】：目的:探討骨形態(tài)發(fā)生蛋白-7(BMP7)基因變異與太原地區(qū)漢族人群代謝綜合征的關(guān)聯(lián)性。方法:采用病例-對(duì)照研究方法,本研究選擇2016.4-2017.1期間就診于山西省人民醫(yī)院門診及住院部的代謝綜合征患者90例,正常體檢人群83例,均為太原地區(qū)漢族人群。在基因庫(kù)NCBI中以NT 011362.11為參考序列選取具有代表性的BMP7中rs6025422、rs17480735基因位點(diǎn),應(yīng)用SNaPshot-PCR技術(shù)在所選太原漢族人群中進(jìn)行基因型鑒定。結(jié)果:1.經(jīng)Hardy-Weinberg平衡檢驗(yàn)發(fā)現(xiàn),位點(diǎn)rs6025422基因型AA、GG、AG的分布與位點(diǎn)rs17480745基因型AA、GG、GA的分布均符合Hardy-Weinberg平衡(P0.05),表示所選人群無(wú)選擇性偏倚。2.代謝綜合征組及對(duì)照組在性別、TC、UA方面差異無(wú)統(tǒng)計(jì)學(xué)意義(P0.05),兩組在年齡、LDL-C、BMI、WHR方面差異有統(tǒng)計(jì)學(xué)意義(P0.05);兩組在基因型rs6025422方面差異有統(tǒng)計(jì)學(xué)意義(P0.05),在基因型rs17480745方面差異無(wú)統(tǒng)計(jì)學(xué)意義(P0.05)。3.以代謝綜合征為因變量,以年齡、BMI、位點(diǎn)rs6025422基因型、LDL-C、WHR為自變量進(jìn)行多因素Logistic回歸分析,年齡、LDL-C和基因型進(jìn)入回歸方程,年齡、LDL-C是代謝綜合征的危險(xiǎn)因素(年齡OR=1.090,95%CI:1.056-1.126,P=0.001;LDL-C OR=1.648,95%CI:1.094-2.484,P=0.049);而在基因型rs6025422中GG型是代謝綜合征的保護(hù)因素(OR=-0.995,95%CI:0.179-0.763,P=0.018)。結(jié)論:1.BMP7基因位點(diǎn)rs6025422的變異與太原地區(qū)漢族人群代謝綜合征有關(guān)聯(lián);2.年齡和LDL-C是太原漢族人群代謝綜合征的危險(xiǎn)因素,rs6025422變異位點(diǎn)的GG基因型是太原漢族人群代謝綜合征的保護(hù)因素。
[Abstract]:Objective: to investigate the association between bone morphogenetic protein-7 (BMP7) gene variation and metabolic syndrome of Han nationality in Taiyuan. Methods: a case-control study was conducted. 90 patients with metabolic syndrome and 83 normal controls were selected from the outpatient and inpatient departments of Shanxi Provincial people's Hospital from June to July 2016.All of them were Han nationality in Taiyuan. The rs6025422 rs17480735 locus of the representative BMP7 was selected in the NCBI gene bank using NT 011362.11 as reference sequence, and the genotypes were identified by SNaPshot-PCR technique in the selected Taiyuan Han population. The result is 1: 1. By Hardy-Weinberg equilibrium test, it was found that the distribution of rs6025422 genotype AA-GGG AG and that of locus rs17480745 genotype AA-GGG GGG G G G of the rs6025422 genotype were in accordance with the Hardy-Weinberg equilibrium P 0.05, indicating that there was no selective bias. There was no significant difference in sex TCU UA between the metabolic syndrome group and the control group (P 0.05), but there was a significant difference between the two groups in age (P 0.05), genotype rs6025422 (P 0.05), and genotype rs17480745 (P 0.05 .3) in the two groups, and there was no significant difference between the two groups in terms of age and age (P < 0. 05, P 0. 05, P 0. 05, P 0. 05, P 0. 05, P 0. 05, P 0. 05, P 0. 05, P 0. 05). Taking metabolic syndrome as dependent variable, age BMIand locus rs6025422 genotype LDL-CHR as independent variable, multivariate Logistic regression analysis was carried out. Age, LDL-C and genotype entered the regression equation. Age LDL-C was the risk factor of metabolic syndrome (CI: 1.056-1.126P0. 001) LDL-C ORB was 1.64895 CI: 1.094-2.484P0.049, while in genotype rs6025422, GG type was the protective factor of metabolic syndrome, OR-0.99595CI0.179-0.763P0.018. Conclusion 1. The variation of rs6025422 in BMP7 gene is associated with metabolic syndrome of Han nationality in Taiyuan area. Age and LDL-C were the risk factors of metabolic syndrome in Taiyuan Han population. The GG genotype of rs6025422 mutation site was the protective factor of metabolic syndrome in Taiyuan Han population.
【學(xué)位授予單位】：山西醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：R589

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