本文選題：菊花 + 低溫�。� 參考：《河南農(nóng)業(yè)大學(xué)學(xué)報(bào)》2017年03期

【摘要】：以秋菊(Chrysanthemum morifolium Ramat.)抗寒品種‘星光燦爛’為材料,利用RT-PCR和RACE方法從葉片中克隆△9硬脂酰-ACP去飽和酶(Stearoyl-ACP desaturase,SAD)基因,命名為CmSAD,Gen Bank登錄號(hào)為KC529335。該基因cDNA全長(zhǎng)1 203 bp,編碼401個(gè)氨基酸,相對(duì)分子質(zhì)量為45.57 k D,等電點(diǎn)為6.48,編碼的氨基酸序列與新疆雪蓮?fù)葱宰罡?80.29%)。通過(guò)對(duì)該蛋白進(jìn)行生物信息學(xué)分析得出其沒(méi)有跨膜結(jié)構(gòu),主要存在于葉綠體基質(zhì)中,N端含有一段包含60個(gè)氨基酸的葉綠體轉(zhuǎn)運(yùn)肽。通過(guò)實(shí)時(shí)熒光定量PCR研究低溫脅迫下葉片和根系中CmSAD基因的表達(dá)量變化,該基因在葉片和根系中均有表達(dá)。根系中CmSAD基因的表達(dá)量隨著溫度的降低而降低,16℃時(shí)表達(dá)量最高;葉片中CmSAD表達(dá)量隨著溫度的降低先升高再降低,5℃時(shí)最高。隨著溫度的降低,菊花葉片和根系中CmSAD基因的表達(dá)情況表現(xiàn)出一定的差異。CmSAD基因的表達(dá)變化與溫度有關(guān),為低溫脅迫下菊花脂肪酸代謝的分子機(jī)理研究提供了基礎(chǔ)。
[Abstract]:Chrysanthemum morifolium Ramat. The gene of 9 stearoyl-ACP desaturase (Stearoyl-ACP desaturase) was cloned from the leaves by RT-PCR and RACE, and named as CmSADGen Bank accession number KC529335. The total length of cDNA is 1 203 BP, encoding 401 amino acids, the relative molecular weight is 45.57 KD, and the isoelectric point is 6.48. The amino acid sequence encoded by this gene has the highest homology with that of Saussurea Sinkiang (80.29). By bioinformatics analysis, it was found that the protein had no transmembrane structure and mainly existed in the chloroplast matrix containing a chloroplast transport peptide containing 60 amino acids. The changes of CmSAD gene expression in leaves and roots under low temperature stress were studied by real-time fluorescence quantitative PCR. The CmSAD gene was expressed in both leaves and roots. The expression of CmSAD gene in roots decreased with the decrease of temperature at 16 鈩，

本文編號(hào)：1873232