本文選題：差異表達 + 轉(zhuǎn)基因��； 參考：《生物工程學報》2017年01期

【摘要】：研究已表明植物特有的一些NAC(NAM,ATAF1/2,CUC2)轉(zhuǎn)錄因子可提高植物抗逆性,利用基因芯片技術篩選轉(zhuǎn)SlNAC1基因擬南芥與野生型擬南芥間差異表達基因,能夠為研究轉(zhuǎn)基因擬南芥非生物脅迫抗性相關基因提供依據(jù)。結果顯示,在轉(zhuǎn)SlNAC1基因擬南芥43 604個基因中有3 046個差異表達2倍以上的基因。對差異表達5倍以上基因經(jīng)過GO富集度統(tǒng)計學分析表明,細胞組分相關基因占33.05%;分子功能相關基因占33.95%;生物學過程相關基因占33.00%。對差異表達2倍以上基因進行KEGG信號通路分析,結果表明有2 431個基因涉及到88個不同的信號通路。通過篩選獲得轉(zhuǎn)基因擬南芥非生物脅迫抗性相關候選基因,為后續(xù)研究NAC轉(zhuǎn)錄因子的下游基因及其調(diào)控網(wǎng)絡的構建提供方向和理論支撐。
[Abstract]:Studies have shown that some plant specific transcription factors of NACMAMN ATAF1 / 2 CUC2 can improve plant stress resistance. Differential expression genes between transgenic Arabidopsis thaliana and wild type Arabidopsis thaliana were screened by gene chip technique. It can provide the basis for the study of transgenic Arabidopsis Arabidopsis abiotic stress resistance related genes. The results showed that 3 046 of 43 604 genes were differentially expressed in Arabidopsis thaliana with SlNAC1 gene. Statistical analysis of differentially expressed genes by go enrichment showed that cell component related genes accounted for 33.05 genes, molecular function-related genes accounted for 33.95%, biological process genes accounted for 33.00%. The KEGG signaling pathway analysis of differentially expressed genes showed that 2 431 genes were involved in 88 different signal pathways. Candidate genes related to abiotic stress resistance of Arabidopsis thaliana were obtained by screening, which provided the direction and theoretical support for further research on downstream genes of NAC transcription factors and the construction of regulatory networks.
【作者單位】： 遼寧師范大學生命科學學院遼寧省植物生物技術重點實驗室;
【基金】：國家自然科學基懶(No.31340052) 遼寧省教育廳科技研究項目(No.L201683655)資助~~
【分類號】：Q943.2
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本文編號：1852607