發(fā)布時間：2018-05-05 12:51

  本文選題：擬南芥 + LRR-RLKs�。� 參考：《核農學報》2017年04期

【摘要】：為研究LRR-RLKs亞家族基因RLK6(AT2G26730)在擬南芥生長發(fā)育過程中的作用,本研究構建了植物過表達載體p Super1300-RLK6,獲得5個過表達突變體,其中2個獨立株系(OE8,OE9)用于表型分析。通過對野生型(WT)、RLK6基因缺失突變體(rlk6-2、rlk6-3和rlk6-6)、過表達株系OE8和OE9生長發(fā)育過程的詳細觀察和分析發(fā)現(xiàn),RLK6基因缺失突變體在整個生長發(fā)育過程中的表型與WT差異不明顯,而過表達株系OE8和OE9比WT開花早。此外,利用實時定量PCR(qRT-PCR)檢測各基因型植株中開花相關基因的表達情況,結果表明,相對于WT,OE8和OE9中的開花促進基因CO、SOC1和LFY的表達量顯著升高,而開花抑制基因FLC的表達量顯著下降。研究結果說明RLK6過表達促進了擬南芥的開花,這為后續(xù)深入研究RLK6的作用機制奠定了基礎。
[Abstract]:In order to study the role of LRR-RLKs subfamily gene RLK6 (AT2G26730) in the growth and development of Arabidopsis thaliana, a plant overexpression vector, p Super1300-RLK6, was constructed, and five overexpression mutants were obtained, among which two independent lines were used for phenotypic analysis. Through the detailed observation and analysis of the growth and development process of the wild-type OE8 and OE9 lines, it was found that there was no significant difference between the phenotypic and WT phenotypes during the whole growth and development process of the wild-type OE8 / RLK6 gene deletion mutants, rlk6-2nrlk6-3 and rlk6-6, respectively. The overexpression lines OE8 and OE9 bloomed earlier than WT. In addition, the expression of flowering related genes in different genotypes was detected by real-time quantitative PCRQRT-PCR. the results showed that the expression of COOSOC1 and LFY in WTOOE8 and OE9 was significantly higher than that in WTOOE8 and OE9. However, the expression of flowering suppressor gene FLC decreased significantly. The results showed that the overexpression of RLK6 promoted the flowering of Arabidopsis thaliana, which laid a foundation for further study on the mechanism of RLK6.
【作者單位】： 河南科技大學農學院;中國農業(yè)大學生物學院/植物生理生化國家重點實驗室;新疆醫(yī)科大學藥學院;
【基金】：國家自然科學基金(31570254) 博士科研啟動基金(09001770)
【分類號】：Q943.2

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