發(fā)布時間：2018-04-29 13:10

  本文選題：食管腫瘤 + 食管癌　； 參考：《山東醫(yī)藥》2017年15期

【摘要】：目的觀察食管鱗癌(ESCC)組織中自噬相關(guān)基因(ATG9B)9B mRNA及蛋白的表達變化,并探討其意義。方法食管癌切除術(shù)中切取的新鮮ESCC組織及癌旁正常黏膜組織各70例份,采用qRT-PCR法檢測ATG9B mRNA。收集198例份食管癌根治切除術(shù)留取的ESCC組織及癌旁正常黏膜組織標本構(gòu)建組織芯片,采用免疫組化法檢測ATG9B蛋白,分析ATG9B蛋白表達與ESCC臨床病理參數(shù)的關(guān)系。結(jié)果 ESCC組織、正常食管黏膜組織中ATG9B mRNA相對表達量分別為0.303±0.197、1.186±0.642,ESCC組織中ATG9B mRNA相對表達量低于正常食管黏膜組織(P0.05)。ESCC組織中ATG9B蛋白陽性表達率低于正常食管黏膜組織(分別為32.6%、60.5%,P0.05)。ATG9B蛋白在高、中、低分化ESCC組織中陽性表達率依次降低,在Ⅰ~Ⅱ期腫瘤組織中的陽性表達率高于Ⅲ~Ⅳ期組織(P均0.05)。結(jié)論 ESCC組織中ATG9B mRNA及蛋白表達均低于正常食管黏膜組織;ATG9B表達下調(diào)可能與ESCC的發(fā)生發(fā)展有關(guān)。
[Abstract]:Objective to investigate the expression of ATG9Bn9B mRNA and protein in esophageal squamous cell carcinoma (ESCC) and its significance. Methods A total of 70 fresh ESCC tissues and 70 adjacent normal mucosa tissues were collected during resection of esophageal carcinoma. ATG9B mRNA was detected by qRT-PCR method. The tissue microarray was constructed from 198 specimens of ESCC tissue and normal mucosa adjacent to esophageal carcinoma after radical resection of esophageal carcinoma. The expression of ATG9B protein was detected by immunohistochemical method. The relationship between the expression of ATG9B protein and the clinicopathological parameters of ESCC was analyzed. Results ESCC tissue, The relative expression of ATG9B mRNA in normal esophageal mucosa was 0.303 鹵0.197 1.186 鹵0.642ESCC was lower than that in normal esophageal mucosal tissue P0.05. ATG9B protein positive rate was lower than that in normal esophageal mucosa tissue (32.60.75%, P 0.05%. ATG9B protein was higher than that in normal esophageal mucosal tissue (32.60.75%, P 0.05%). ATG9B protein in normal esophageal mucosa tissue was higher than that in normal esophageal mucosal tissue (32.60.75%, P 0.05 .ATG9B protein, respectively). The positive expression rate of ESCC in stage 鈪，

本文編號：1820071