本文選題：癌 + 口腔��； 參考：《重慶醫(yī)科大學(xué)》2017年碩士論文

【摘要】：目的探討人口腔鱗癌SCC15細(xì)胞中生物鐘基因PER2的表達(dá)改變對(duì)細(xì)胞增殖、凋亡以及其它生物鐘基因的影響。方法通過構(gòu)建shRNA干擾人口腔鱗癌SCC15細(xì)胞中PER2基因;應(yīng)用流式細(xì)胞儀檢測(cè)口腔鱗癌細(xì)胞的增殖和凋亡水平的改變情況;實(shí)時(shí)熒光定量PCR檢測(cè)生物鐘基因CLOCK、BMAL1、PER1、PER3、DEC1、DEC2、CRY1、CRY2、TIM、CKIε、RORα、NPAS2和REV-ERBαmRNA表達(dá)。結(jié)果PER2沉默之后,SCC15癌細(xì)胞的凋亡降低,增殖加強(qiáng)(P0.05);SCC15細(xì)胞中生物鐘基因PER3、BMAL1、DEC1、DEC2、CRY2、TIM、RORα和NPAS2 mRNA的表達(dá)水平顯著降低,PER1和REV-ERBαmRNA的表達(dá)顯著升高(P0.05)。結(jié)論在癌細(xì)胞中,生物鐘基因PER2對(duì)生物鐘基因網(wǎng)絡(luò)中其它生物鐘基因具有重要調(diào)控作用,PER2表達(dá)降低導(dǎo)致細(xì)胞增殖增加和凋亡水平下降。
[Abstract]:Objective to investigate the effect of PER2 expression on cell proliferation, apoptosis and other clock genes in human oral squamous cell carcinoma (SCC15) cells. Methods shRNA was constructed to interfere with the expression of PER2 gene in human oral squamous cell carcinoma (SCC15) cells, flow cytometry was used to detect the changes of cell proliferation and apoptosis in oral squamous carcinoma cells, and real-time quantitative PCR was used to detect the expression of REV-ERB 偽 -NPAS2 and REV-ERB 偽 mRNA in human oral squamous cell carcinoma cells. Results after PER2 silencing, the apoptosis of SCC15 cells was decreased, and the expression of clock gene PER3, BMAL1, DEC2, CRY2, TIMROR 偽 and NPAS2 mRNA in SCC15 cells was significantly decreased. The expression of REV-ERB 偽 and PER1 was significantly increased in SCC15 cells. Conclusion in cancer cells, PER2 plays an important role in regulating the expression of other clock genes in the biological clock gene network. The decrease of PER2 expression leads to the increase of cell proliferation and the decrease of apoptosis.
【學(xué)位授予單位】：重慶醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：R739.8

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相關(guān)期刊論文 前3條

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本文編號(hào)：1796851