發(fā)布時(shí)間：2018-04-19 13:24

  本文選題：水產(chǎn)品微生物 + 抗生素抗性基因��； 參考：《現(xiàn)代食品科技》2017年05期

【摘要】：論文選取廣州市天河區(qū)兩家菜市場(chǎng)、一家海鮮市場(chǎng)和三家超市的48份水產(chǎn)品為研究對(duì)象,提取了三種不同水產(chǎn)品:蝦、魚和貝類的總DNA,采用實(shí)時(shí)熒光定量PCR(Quantitative Real-time PCR)法,對(duì)市售水產(chǎn)品中6類抗生素抗性基因(Antibiotic Resistance Genes,ARGs):tet A、tet B、tet M、sulⅡ、flo R、aph A-1、aad A、erm B、cml A和Ⅰ類整合子5’端整合酶基因int I1、3’端磺胺耐藥基因sulⅠ、季銨鹽化合物及溴化乙錠的耐藥基因qac EΔ1進(jìn)行定量。研究顯示:6類ARGs及Ⅰ類整合子基因在水產(chǎn)品中均有檢出,基因拷貝數(shù)大小從10~2~10~8跨越七個(gè)數(shù)量級(jí),最小值為1.86×10~2 copies/g,最大值為8.98×10~8 copies/g。三種水產(chǎn)品蝦、魚、貝類中,蝦類的ARGs含量最高,貝類最低;三類采樣地點(diǎn)中,菜市場(chǎng)來源的水產(chǎn)品中ARGs含量最高,海鮮市場(chǎng)來源的次之,超市來源的最低。本研究為進(jìn)一步探索ARGs和可移動(dòng)耐藥元件整合子介導(dǎo)的細(xì)菌耐藥性傳播而引起的食源性疾病機(jī)理奠定了基礎(chǔ)。
[Abstract]:In this paper, 48 samples of aquatic products from two vegetable markets, one seafood market and three supermarkets in Tianhe District of Guangzhou City were selected as research objects. The total DNA of three different aquatic products, shrimp, fish and shellfish, was extracted by real-time fluorescence quantitative PCR(Quantitative Real-time method.Six antibiotic resistant genes, Antibiotic Resistance Genesus and sul 鈪，

本文編號(hào)：1773273