本文選題：蛋雞 + 卵泡��； 參考：《畜牧獸醫(yī)學報》2016年03期

【摘要】：旨在研究雙鏈RNA(Double-stranded RNA,dsRNA)對海蘭褐蛋雞卵泡可卡因-苯丙胺調節(jié)轉錄肽(Cocaine-and amphetamine-regulated transcript,CART)基因表達及雌激素(Estrodiol,E2)和孕酮(Progestin,P)分泌的影響。選擇60只20周齡海蘭褐蛋雞,全價日糧預飼30d后,隨機平均分為3個處理組:對照組(A組)、dsRNA每5d注射1次(B組)、dsRNA僅第1天注射1次(C組);30d后每組各選擇10只健康蛋雞宰殺,分別采集3~5、6~8、9~12mm卵泡,提取總RNA進行qRT-PCR檢測;并對E2和P濃度進行測定。結果顯示:注射dsRNA后,處理組B和C組CART表達量與對照組相比均降低,且在6~8 mm卵泡CART表達量顯著低于對照組(P0.05);B組注射dsRNA后,可顯著提高6~8、9~12mm卵泡E2的分泌(P0.05);但各處理組對各卵泡P濃度無顯著影響。表明體外靜脈注射dsRNA可抑制蛋雞卵泡CART的表達,并進一步通過促進卵泡細胞E2的分泌來影響卵泡的發(fā)育。
[Abstract]:Sixty 20-week-old Hailan brown laying hens were randomly divided into three treatment groups after 30 days of prefeeding the whole ration: control group A was treated with dsRNA once every 5 days and group B was treated with dsRNA once every 5 days. After 30 days of treatment, 10 healthy laying hens were slaughtered in each group.The follicles were collected from the follicles of 3 pieces and 5 pieces, and the total RNA was extracted for qRT-PCR detection, and the concentrations of E 2 and P were determined.The results showed that the expression of CART in group B and C was significantly lower than that in group B and C after injection of dsRNA, and the expression of CART in 6mm follicle was significantly lower than that in group B (P 0.05).The E _ 2 secretion of 12 mm follicles increased significantly (P 0.05), but there was no significant effect on the P concentration of each follicle in each treatment group.The results showed that dsRNA could inhibit the expression of CART and further affect the development of follicles by promoting the secretion of E2 in follicle cells.
【作者單位】： 山西農業(yè)大學生命科學學院;山西農業(yè)大學動物科技學院;
【基金】：國家自然科學基金(31172211) 農業(yè)部948項目(2010-Z43) 山西省橫向協(xié)作與委托項目(2010HX54) 山西省回國留學人員科研資助項目(2014-重點5) 山西省科技攻關項目(20130311027-2) 山西省人事廳人才引進項目 山西農業(yè)大學引進人才博士科研啟動費(2014ZZ04) 科研管理費資助重大項目和標志性成果培育項目(71060003)
【分類號】：S831

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