本文選題：輸入性卵形瘧　切入點：卵形瘧原蟲curtisi亞種　出處：《中國寄生蟲學與寄生蟲病雜志》2017年01期

【摘要】：目的分析河南省2015年輸入性卵形瘧原蟲富色氨酸抗原(Plasmodium ovale tryptophan-rich antigen,Po TRA)基因序列。方法采集河南省2015年22例輸入性卵形瘧患者的血樣,收集患者相關(guān)信息。提取血樣DNA,用巢式PCR方法擴增Po TRA基因,連接至p MD18-T載體,提取質(zhì)粒進行測序,在Gen Bank中進行同源性比對,判斷卵形瘧原蟲的亞種,并對其基因長度及種類進行分析。對Po TRA基因的氨基酸序列進行比對,分析氨基酸的差異。用鄰接法構(gòu)建系統(tǒng)進化樹,分析樣本間的親緣關(guān)系。結(jié)果 22例卵形瘧中,8例為卵形瘧原蟲wallikeri亞種(P.ovale wallikeri),占36.4%,Po TRA基因長度有245和299 bp 2種類型,以245 bp為主;14例為卵形瘧原蟲curtisi亞種(P.ovale curtisi),占63.6%,Po TRA基因長度有299、317和335 bp等3種類型,以299 bp為主。氨基酸序列比對顯示,wallikeri亞種的2種基因類型相差2個氨基酸單元(MANPINMANPIN和AITPIN),curtisi亞種的3種基因類型間相差2個氨基酸單元(TITPIS和TINPIN)。系統(tǒng)進化樹顯示,22例樣本分為curtisi和wallikeri 2個亞群,其中curtisi亞群又分為2個亞支,299 bp的樣本在同一亞支內(nèi),317和335 bp的樣本在另一亞支內(nèi),親緣關(guān)系更近。結(jié)論 2015年河南省輸入性卵形瘧有curtisi和wallikeri 2個亞種,其Po TRA基因存在多態(tài)性,curtisi亞種有3種基因類型,wallikeri亞種有2種基因類型。
[Abstract]:Objective to analyze the sequence of Plasmodium ovale tryptophan-rich antigenn Po TRAA gene of imported Plasmodium ovale tryptophan-rich in Henan Province in 2015.Methods Blood samples were collected from 22 imported malaria patients in Henan province in 2015.The Po TRA gene was amplified by nested PCR method and ligated to p MD18-T vector. The plasmid was sequenced. The homology was compared in Gen Bank to determine the subspecies of Plasmodium ovum, and the gene length and species were analyzed.The amino acid sequence of Po TRA gene was compared and the difference of amino acids was analyzed.The phylogenetic tree was constructed by adjacency method, and the relationship between samples was analyzed.Results among the 22 cases of Plasmodium ovum, 8 cases were P.ovale wallikeriana, accounting for 36.4 BP and 299bp in length, and 14 cases were curtisi subspecies of P.ovale curtisii, accounting for 299317 BP and 335bp in length.299bp was dominant.The amino acid sequence alignment showed that there were 2 amino acid units difference between the two gene types of wallikeri subspecies, MANPINMANPIN and the three gene types of AITPINI subspecies, two amino acid units, TITPIS and TINPINN.The phylogenetic tree showed that 22 samples were divided into two subgroups, curtisi and wallikeri, in which the curtisi subgroup was further divided into two subgroups, the samples with 299bp in the same subbranch and 335bp samples in the same subbranch were more closely related to each other.Conclusion in 2015, there were two subspecies of curtisi and wallikeri in imported oval malaria in Henan Province. The Po TRA gene was polymorphic.
【作者單位】： 河南省疾病預(yù)防控制中心;
【基金】：河南省醫(yī)學科技攻關(guān)計劃項目(No.201304053,201602318)~~
【分類號】：R531.3

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