本文選題：GhCOMT　切入點：細胞壁　出處：《分子植物育種》2017年06期

【摘要】：為了深入研究GhCOMT1基因的功能,確定GhCOMT1基因?qū)γ蘩w維細胞壁增厚的影響。本實驗通過構(gòu)建棉花木質(zhì)素合成途徑中的咖啡酸-O-甲基轉(zhuǎn)移酶基因GhCOMT1的瞬時表達載體,用將pRTL2-GUS/NIa瞬時表達空載體連接GhCOMT1基因的目的片段,獲得pGUS-GhCOMT1融合表達載體,并將其轉(zhuǎn)化到棉花胚珠表皮細胞中進行表達。通過檢測可以發(fā)現(xiàn)pGUS-GhCOMT1融合表達載體在洋蔥表皮細胞及棉花胚珠表皮細胞中高效表達,表明棉花GhCOMT1基因?qū)γ蘩w維的的伸長有顯著的影響,同時為研究棉花纖維發(fā)育調(diào)控的分子機理提供理論基礎(chǔ)。
[Abstract]:In order to study the function of GhCOMT1 gene, the effect of GhCOMT1 gene on cell wall thickening of cotton fiber was determined.In this experiment, the transient expression vector of caffeic acid-O-methyltransferase (GhCOMT1) gene in cotton lignin synthesis pathway was constructed, and the target fragment of GhCOMT1 gene was ligated with pRTL2-GUS/NIa transient expression empty vector. The fusion expression vector of pGUS-GhCOMT1 was obtained.It was transformed into cotton ovule epidermis cells for expression.It was found that pGUS-GhCOMT1 fusion expression vector was highly expressed in onion epidermis cells and cotton ovule epidermis cells, indicating that cotton GhCOMT1 gene had a significant effect on cotton fiber elongation.At the same time, it provides a theoretical basis for studying the molecular mechanism of cotton fiber development and regulation.
【作者單位】： 新疆農(nóng)業(yè)科學院核技術(shù)生物技術(shù)研究所;
【基金】：新疆維吾爾自治區(qū)自然科學基金青年科學基金(2016D01B029)資助
【分類號】：Q943.2;S562
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本文編號：1717524