發(fā)布時間：2018-03-13 07:49

  本文選題：ABO亞型　切入點：等位基因　出處：《天津醫(yī)科大學》2017年碩士論文　論文類型：學位論文

【摘要】：目的:通過對濱海新區(qū)無償獻血人群ABO血型的血清學和分子生物學檢測,掌握ABO亞型的血清學表現(xiàn),并對ABO亞型等位基因突變位點進行分析。方法:使用ABO血型常規(guī)檢測法對無償獻血者進行ABO血型初篩,發(fā)現(xiàn)正反定型不符的疑難血型;使用經(jīng)典鹽水試管法對正反定型不符標本進行ABO亞型鑒定;將血清學鑒定為ABO亞型的標本送往生工生物工程(上海)股份有限公司進行測序;對測序結果顯示存在錯義突變者進行蛋白質功能分析并使用PyMOL建立糖基轉移酶空間模型。結果:45880名無償獻血者中初篩檢測出正反定型不符標本,經(jīng)進一步鑒定有20例符合ABO亞型血清學特征,分別為A2(1)、AX(2)、Bx(3)、Bm(1)、B3(1)、A2B(2)、AXB(4)、ABx(3)、B(A)(2),cisAB(1)。20例標本經(jīng)過測序分析發(fā)現(xiàn)10個ABO等位基因錯義突變,分別為1009AG、905 AG、940AG、503GT、28GA、588CG、550GA、700CG、640AG、803GC,并造成10個氨基酸置換,分別為R337G、D302G、K314E、R168L、G10R、C196W、V184M、P234A、M214V、G268A,對應的10個亞型等位基因分別為A205、AX18、AX13、Bw22、B310、Bx04、Bx08、B(A)02、B(A)04、cisAB01。錯義突變導致的氨基酸置換在進化中為保守氨基酸,通過空間模型分析顯示氨基酸置換前后其側鏈的空間構象發(fā)生較大改變。結論:(1)ABO亞型在血清學檢測中常出現(xiàn)異常減少和(或)增加的抗原和(或)抗體,造成血型檢測正反定型不符,需掌握ABO亞型血清學特性以做出快速而準確的血型鑒定。(2)ABO等位基因由于發(fā)生錯義突變而導致A或B糖基轉移酶發(fā)生氨基酸置換,氨基酸置換后由于物理化學性質、帶電性質和疏水性質以及氨基酸側鏈間分子間作用力的改變而影響A或B糖基轉移酶的催化活性和特異性,從而形成ABO亞型表型。
[Abstract]:Objective: to study the serological and molecular manifestations of ABO blood group in the population of free blood donation in Binhai New area, and to understand the serological manifestation of ABO subtype. The allelic mutation sites of ABO subtype were analyzed. Methods: the ABO blood group of unpaid blood donors was screened by routine detection of ABO blood group, and the difficult blood group was found to be not consistent with positive and negative typing. The ABO subtypes of positive and negative unmatched samples were identified by the classical salt water test tube method, and the samples identified as ABO subtypes were sent to bioengineering (Shanghai) Co., Ltd for sequencing. The results of sequencing showed that the missense mutant was used to analyze the protein function and the spatial model of glycosyltransferase was established by PyMOL. After further identification of 20 cases of ABO subtype serological characteristics, they were identified as A2G905 AX905 AG9AG905 AG905 AG905 AG905 AG503GT28GA588CG550GA550GA550GA700 CG640GCC, and 10 ABO allele missense mutations were found by sequencing analysis of 10 ABO allele missense mutations, respectively, after sequencing analysis, we found 10 ABO alleles missense mutations, respectively, which were AG905, AG905, AG905, AG905, AG503, GT28, AG503, GT288CG5, CG550GA550GA700, CG640GCC, and resulted in 10 amino acid replacements. The corresponding 10 alleles are A205AX18AX18AX13AX13B310Bx04BX010BX04BX04BX01. the amino acid replacement caused by missense mutation is conserved in evolution, and the corresponding 10 alleles are A205AX18AX18AX13AX13B310Bx04BX08BX08BX08BX02A02A04A01. the amino acid replacement caused by missense mutation is conserved in evolution, and the corresponding 10 alleles are A205AX18AX13AX13AX13B310Bx04BX08BX08BX08BX08BX02A02A01. The spatial model analysis showed that the spatial conformation of the side chain of Amino acid was changed greatly before and after amino acid replacement. Conclusion the abnormal reduction and / or increase of antigens and / or antibodies are often found in the serological examination of the ABO subtype. It is necessary to master the serological characteristics of ABO subtype in order to make rapid and accurate blood type identification. The missense mutation of the allele causes amino acid replacement of A or B glycosyltransferase. After the replacement of amino acids, the catalytic activity and specificity of A or B glycosyltransferases were affected by the changes of physicochemical, charged and hydrophobic properties and the intermolecular forces between amino acids, thus forming the phenotype of ABO subtype.
【學位授予單位】：天津醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：R457.11

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