本文關鍵詞： 隱睪 Nme 凋亡　出處：《華中科技大學學報(醫(yī)學版)》2017年01期 　論文類型：期刊論文

【摘要】：目的觀察氧化應激相關基因Nme5在隱睪中的表達變化,探討其在生精細胞凋亡中的作用。方法建立單側隱睪小鼠模型,采用TUNEL結合形態(tài)學觀察確認小鼠隱睪模型是否建立成功并分析生精細胞凋亡情況。采用Realtime PCR,Western blot以及免疫組化分析隱睪手術后第1、2、3、4、5、6、9和15天與正常側相比隱睪側氧化應激相關基因Nme5 mRNA和蛋白表達情況。結果免疫組化結果顯示正常睪丸組織中,Nme5在生精上皮周期的Ⅱ~Ⅻ階段均表達于粗線期初級精母細胞、圓形精子以及長形精子中;TUNEL結合形態(tài)學觀察表明小鼠隱睪模型構建成功;Realtime PCR結果顯示術后第4天Nme5 mRNA表達量輕微上升,第6天顯著下降;Western blot結果顯示術后第4天Nme5蛋白開始下降,至第9天急劇降低;免疫組化結果顯示術后第4~6天隱睪側Nme5在多核巨細胞中表達上調。結論正常睪丸組織中Nme5在粗線期初級精母細胞、圓形精子以及長形精子中都有表達,參與了從減數(shù)分裂到精子變形的全部過程,在精子發(fā)生中起重要作用。隱睪狀態(tài)下Nme5在新生圓形精子和由退行性圓形精子細胞組成的多核巨細胞中表達上調,可能通過調控氧化應激相關基因Gpx5來調控圓形精子細胞凋亡。
[Abstract]:Objective to investigate the expression of oxidative stress related gene Nme5 in cryptorchidism and its role in spermatogenic cell apoptosis. TUNEL combined with morphological observation was used to confirm the success of mouse cryptorchidism model and to analyze the apoptosis of spermatogenic cells. The oxidation of cryptorchidism was analyzed by Realtime blot and immunohistochemistry analysis on the 1st and 15th day after cryptorchidism operation. Results Immunohistochemical results showed that nme5 was expressed in primary spermatocytes in the stage 鈪，

本文編號：1528334