本文關(guān)鍵詞： 水稻 HSP90 基因家族 表達(dá) 逆境　出處：《四川農(nóng)業(yè)大學(xué)》2016年博士論文　論文類型：學(xué)位論文

【摘要】：隨著近年來極端天氣的出現(xiàn)愈加頻繁,高溫、低溫、干旱及土壤鹽漬化等逆境脅迫對(duì)作物的生長(zhǎng)、發(fā)育、產(chǎn)量的影響也越發(fā)凸顯。研究作物耐逆機(jī)制,培育耐逆品種成為當(dāng)前亟待解決的問題。HSP90家族蛋白是一類豐富的分子伴侶蛋白,并廣泛存在于真核生物中的保守蛋白。HSP90家族蛋白的主要功能主要是協(xié)助其它蛋白的正確折疊,并在逆境應(yīng)答網(wǎng)絡(luò)中發(fā)揮重要作用。本實(shí)驗(yàn)中利用生物信息學(xué)的工具,發(fā)現(xiàn)并分析了日本晴中HSP90家族的9個(gè)OsHSP90蛋白(OsHSP90-1至OsHSP90-9).通過序列比對(duì),我們?cè)谒綡SP90家族蛋白中確定了三個(gè)基序。通過進(jìn)化樹分析,將水稻HSP90家族蛋白分為三簇(Ⅰ,Ⅱ,Ⅲ)。本實(shí)驗(yàn)在水稻10個(gè)組織中對(duì)這9個(gè)OsHSP90蛋白基因進(jìn)行了表達(dá)量分析。結(jié)果表明在10個(gè)部位的組織中OsHSP90-4、OsHSP90-6和OsHSP90-7有較高的表達(dá)水平,而OsHSP90-5和OsHSP90-8的表達(dá)水平非常低。OsHSP90-1只特異性的在開花14天后的胚中表達(dá)。據(jù)文獻(xiàn)報(bào)道,在熱激和其它逆境脅迫下,熱激蛋白的表達(dá)會(huì)受到正向調(diào)控。但在本研究中,不同逆境脅迫條件下,水稻OsHSP90家族蛋白的表達(dá)模式卻是各式各樣的。在干旱、高鹽、低溫、熱激脅迫下OsHSP90-2和OsHSP90-4的表達(dá)受到了正向調(diào)控,在高鹽、干旱的脅迫下OsHSP90-3和OsHSP90-5的表達(dá)受到了負(fù)向調(diào)控。在干旱脅迫下OsHSP90-7和OsHSP90-9受到負(fù)向調(diào)控。與對(duì)照相比,在多種逆境脅迫下OsHSP90-6的表達(dá)量基本保持不變。我們分別用OsHSP90-2和OsHSP90-4轉(zhuǎn)化大腸桿菌,均能提高轉(zhuǎn)化菌株的細(xì)胞活性并提高對(duì)高溫、高鹽、干旱逆境環(huán)境的耐受力。我們分別用OsHsP90-2和OSHsP90-4轉(zhuǎn)化水稻,但只有OsHSP90-4的水稻轉(zhuǎn)化子對(duì)高溫的耐受力有明顯提高并增強(qiáng)水稻轉(zhuǎn)化子對(duì)ABA的敏感性。這些結(jié)果為進(jìn)一步研究水稻OsHSP90基因家族提供了新的視角。
[Abstract]:With the increasing occurrence of extreme weather in recent years, high temperature, low temperature, drought and soil salinization and other stresses on crop growth, development, yield has become more and more prominent. Breeding of stress tolerance varieties has become a problem to be solved. HSP90 family protein is a kind of abundant molecular chaperone protein. The main function of the conserved protein. HSP90 family, which is widely present in eukaryotes, is to assist in the correct folding of other proteins. And play an important role in adversity response network. In this experiment, bioinformatics tools are used. Nine OsHSP90 proteins from the HSP90 family in Nippon were found and analyzed by sequence alignment from OsHSP90-1 to OsHSP90-9. We identified three motifs in rice HSP90 family proteins. By phylogenetic tree analysis, rice HSP90 family proteins were divided into three clusters (鈪，

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