本文關(guān)鍵詞： 流感嗜血桿菌 耐藥性 ftsI基因　出處：《中國感染與化療雜志》2017年05期 　論文類型：期刊論文

【摘要】：目的研究兒童呼吸道分離流感嗜血桿菌的耐藥性和該菌ftsI基因分型與耐藥表型的關(guān)系。方法收集2016年第一季度住院患兒鼻咽吸出物中分離到的流感嗜血桿菌141株;用紙片擴散法檢測細菌對抗菌藥物的耐藥性;用Nitrocefin紙片法檢測細菌的β內(nèi)酰胺酶;用PCR技術(shù)對分離菌株進行ftsI基因檢測;比較不同基因型菌株對抗菌藥物的耐藥情況。結(jié)果141株流感嗜血桿菌β內(nèi)酰胺酶檢出率為40.4%(57/141)、氨芐西林耐藥率為53.2%(75/141)。檢出ftsI基因突變率為72.3%(102/141),以Ⅲ型為主(72/102,70.6%)。β內(nèi)酰胺酶基因陰性氨芐西林耐藥型菌株(g BLNAR)對氨芐西林和頭孢呋辛的耐藥率高于β內(nèi)酰胺酶基因陰性氨芐西林敏感型菌株(g BLNAS)(P0.05)。結(jié)論兒童呼吸道分離流感嗜血桿菌ftsI基因突變率高,以Ⅲ型為主。ftsI基因突變增加了流感嗜血桿菌對氨芐西林和頭孢呋辛的耐藥性。
[Abstract]:Objective to study the drug resistance of Haemophilus influenzae isolated from respiratory tract in children and the relationship between ftsI genotyping and drug resistance phenotype of Haemophilus influenzae in children. Methods Influenza isolated from nasopharyngeal aspirates of hospitalized children in in the first quarter of 2016 was collected. 141 strains of Haemophilus; The drug resistance of bacteria to antimicrobial agents was detected by disk diffusion method. The 尾 -lactamases of bacteria were detected by Nitrocefin disk method. PCR technique was used to detect the ftsI gene of isolated strains. Results the detection rate of 尾 -lactamases in 141 strains of Haemophilus influenzae was 40.4% or 57 / 141). The resistant rate of ampicillin was 53.2% and 75 / 141% respectively. The mutation rate of ftsI gene was 72.3% and 102% / 141% respectively. The resistant rate of ampicillin and cefuroxime resistant strains with 尾 lactamase gene negative ampicillin was higher than that of 尾 lactamase gene negative ampicillin sensitive strain (. Conclusion the mutation rate of ftsI gene of Haemophilus influenzae isolated from children's respiratory tract is high. The mutation of type 鈪，

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