本文關鍵詞：豬NR4A1基因的克隆、序列分析及表達模式研究　出處：《西北農林科技大學學報(自然科學版)》2016年01期 　論文類型：期刊論文

  更多相關文章： 豬 NRA 序列分析 表達模式

【摘要】：【目的】克隆豬核受體4A1(Nuclear receptor subfamily 4,group A,member 1,NR4A1)DNA序列全長,并對CDS序列及其表達模式進行分析�！痉椒ā恳晕闯赡觊L大母豬的卵巢為材料,采用電子克隆技術,對豬NR4A1DNA序列進行分離和測序,并對豬NR4A1基因序列進行生物信息學分析;利用性腺激素(人絨毛膜促性腺激素(Human chorionic gonadotropin,HCG)和孕馬血清促性腺激素(Pregnant mare serum gonadotropin,PMSG))處理卵巢顆粒細胞,采用RT-PCR方法分析豬NR4A1基因的表達模式�！窘Y果】獲得豬NR4A1基因4 870bp的DNA序列,其中CDS全長序列為1 734bp,共編碼572個氨基酸;豬NR4A1基因編碼的氨基酸序列與人的對應氨基酸序列的相似性最高(97%),其次為小鼠(94%);Expasy軟件預測結果表明,NR4A1蛋白的氨基酸序列非常保守,其中包含2個鋅指特征(NR C4-type),一個是激素受體超家族,另一個是Zf-C4超家族;豬卵巢顆粒細胞在性腺激素處理后,可誘導豬NR4A1基因在卵泡發(fā)育和排卵時期瞬時表達�！窘Y論】豬NR4A1基因的CDS區(qū)在物種間保守性較強,推測豬NR4A1基因調節(jié)卵泡的發(fā)育和排卵過程。
[Abstract]:[objective] to clone nuclear receptor subfamily group member 1 from porcine nuclear receptor 4A1. The NR4A1)DNA sequence was full-length, and the CDS sequence and its expression pattern were analyzed. [methods] the ovaries of adult sows were used as materials, and the technique of electronic cloning was used. The porcine NR4A1DNA sequence was isolated and sequenced, and the porcine NR4A1 gene sequence was analyzed by bioinformatics. Human chorionic gonadotropin was used as human chorionic gonadotropin. HCG) and Pregnant mare serum gonadotropin (PMSG) were used to treat ovarian granulosa cells. The expression pattern of porcine NR4A1 gene was analyzed by RT-PCR method. [results] the DNA sequence of porcine NR4A1 gene was 4870 BP. The full-length CDS sequence was 1 734 BP, encoding 572 amino acids. The amino acid sequence encoded by porcine NR4A1 gene was similar to the corresponding amino acid sequence of human being, and the highest similarity was found between the amino acid sequence of pig NR4A1 gene and the corresponding amino acid sequence of human. The predicted results of Expasy software showed that the amino acid sequence of NR4A1 protein was very conserved, which contained two zinc finger features NR C4-type, one of which was a hormone receptor superfamily. The other is the Zf-C4 superfamily; Porcine ovarian granulosa cells treated with gonadal hormone could induce the transient expression of porcine NR4A1 gene during follicular development and ovulation. [conclusion] the CDS region of porcine NR4A1 gene is more conserved among species. It is inferred that porcine NR4A1 gene regulates follicle development and ovulation.
【作者單位】： 安徽省農業(yè)科學院畜牧獸醫(yī)研究所;華中農業(yè)大學動物科學院;
【基金】：安徽省農業(yè)科學院科技創(chuàng)新團隊項目(13C0405);安徽省農業(yè)科學院院長青年基金項目(14B0427)
【分類號】：S828
【正文快照】： NR4A1基因作為一個孤核受體的轉錄因子,編碼類固醇-甲狀腺激素超家族。NR4A1蛋白是一類配體依賴轉錄調節(jié)蛋白[1],它在轉錄過程中起作用的是N-末端,參與調節(jié)核定位和轉錄激活過程的是C-末端[2-4]。在細胞中進行的類固醇合成活性研究結果顯示,NR4A1基因表達與P450膽固醇側鏈裂解

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