本文關鍵詞：不同環(huán)境因子對海洋細菌Pseudoalteromonas issachenkoniiHZ引導糖基轉移酶基因表達的影響　出處：《食品工業(yè)科技》2016年22期 　論文類型：期刊論文

  更多相關文章： 海洋細菌Pseudoalteromonas issachenkonii HZ 引導糖基轉移酶 實時熒光定量PCR

【摘要】：為了研究海洋細菌Pseudoalteromonas issachenkonii HZ所產多糖的生物合成基因簇,首先需要克隆到在多糖合成中起關鍵作用的引導糖基轉移酶基因。通過設計引物,采用PCR技術從海洋細菌Pseudoalteromonas issachenkonii HZ成功克隆到該基因,并通過實時熒光定量PCR技術檢測了溫度、p H及海鹽濃度對引導糖基轉移酶基因表達量的影響。結果表明:低溫有利于引導糖基轉移酶的表達,隨著溫度的上升,引導糖基轉移酶基因的表達量先增后減,在20 h時,引導糖基轉移酶的表達量急劇上升,15℃和35℃引導糖基轉移酶表達量分別是20℃表達量的5.2倍、5.8倍;海鹽濃度為4.5%時引導糖基轉移酶的表達量為海鹽濃度3.5%的2.5倍,海鹽濃度為5.5%時引導糖基轉移酶的表達量為海鹽濃度3.5%的2.0倍;10 h時p H為8、9的培養(yǎng)基中引導糖基轉移酶的表達量分別為p H為7的1.64和1.67倍。該結果為探究菌體環(huán)境適應性機制提供了一定的基礎。
[Abstract]:In order to study the biosynthesis gene cluster of polysaccharides produced by marine bacteria Pseudoalteromonas issachenkonii HZ. First of all, we need to clone the leading glycosyltransferase gene which plays a key role in the synthesis of polysaccharides. The gene was cloned successfully from marine bacteria Pseudoalteromonas issachenkonii HZ by PCR. The effects of temperature pH and sea salt concentration on the gene expression of guided glycosyltransferase were detected by real-time fluorescence quantitative PCR. The results showed that low temperature was beneficial to the expression of glycosyltransferase. With the increase of temperature, the expression of leading glycosyltransferase gene increased first and then decreased. At 20 h, the expression of guided glycosyltransferase increased sharply. The expression of glycosyltransferase at 15 鈩，

本文編號：1380611