基于寡核苷酸鏈的汞離子熒光生物傳感器
發(fā)布時間：2019-06-07 11:28
【摘要】：基于G-四鏈體結構和卟啉類化合物N-甲基卟啉二丙酸IX(NMM)結合產生強烈的熒光,利用T-Hg(Ⅱ)-T錯配對汞離子(Hg~(2+))的特異性識別,建立了一種簡單、靈敏、高效的Hg~(2+)檢測新方法。在富含鳥嘌呤(G)寡核苷酸鏈中,引入了大量胸腺嘧啶(T)。在沒有Hg~(2+)存在時,可以自發(fā)形成G-四鏈體結構,與NMM結合產生強烈的熒光;在Hg~(2+)存在時,可與另一條富含T序列的互補鏈通過T-Hg(Ⅱ)-T特異性結合,形成雙鏈DNA分子,從而導致G-四鏈體結構不能產生。優(yōu)化后最佳實驗條件為:緩沖溶液的pH=6.7,20 mmol/L KCl,2.5μmol/L NMM,反應時間為2 h。在優(yōu)化條件下,體系的熒光強度變化值與Hg~(2+)濃度呈現(xiàn)良好的線性關系,線性范圍為50~1000 nmol/L,檢出限為22.8 nmol/L(3σ)。此生物熒光傳感器對Hg~(2+)具有良好的選擇性。實際水樣中Hg~(2+)的加標回收率為106.1%~107.8%,可以滿足實際水樣品中Hg~(2+)的檢測要求。
[Abstract]:Based on the strong fluorescence produced by the binding of G-tetrachain structure and N-methylporphyrin dipropionic acid IX (NMM) to produce strong fluorescence, a simple method was established by using the specific recognition of mercury ion (Hg~ (2) by T-Hg (II)-T mismatch. A new sensitive and efficient method for Hg~ (2) detection. A large amount of thymidine (T). Was introduced into guanine-rich (G) oligodeoxynucleotides. In the absence of Hg~ (2), the G-quadruchain structure can be formed spontaneously and bind to NMM to produce strong fluorescence. In the presence of Hg~ (2), it can bind specifically to another complementary chain rich in T sequence through T-Hg (II)-T to form double-stranded DNA molecule, which leads to the inability of G-quadruchain structure. The optimum experimental conditions were as follows: the reaction time of pH=6.7,20 mmol/L KCl, 2.5 渭 mol / L NMM, was 2 h. Under the optimized conditions, the fluorescence intensity of the system has a good linear relationship with the concentration of Hg~ (2), and the linear range is 50 鈮，
本文編號：2494759
[Abstract]:Based on the strong fluorescence produced by the binding of G-tetrachain structure and N-methylporphyrin dipropionic acid IX (NMM) to produce strong fluorescence, a simple method was established by using the specific recognition of mercury ion (Hg~ (2) by T-Hg (II)-T mismatch. A new sensitive and efficient method for Hg~ (2) detection. A large amount of thymidine (T). Was introduced into guanine-rich (G) oligodeoxynucleotides. In the absence of Hg~ (2), the G-quadruchain structure can be formed spontaneously and bind to NMM to produce strong fluorescence. In the presence of Hg~ (2), it can bind specifically to another complementary chain rich in T sequence through T-Hg (II)-T to form double-stranded DNA molecule, which leads to the inability of G-quadruchain structure. The optimum experimental conditions were as follows: the reaction time of pH=6.7,20 mmol/L KCl, 2.5 渭 mol / L NMM, was 2 h. Under the optimized conditions, the fluorescence intensity of the system has a good linear relationship with the concentration of Hg~ (2), and the linear range is 50 鈮，
本文編號：2494759
本文鏈接：http://www.sikaile.net/kejilunwen/huaxue/2494759.html
教材專著
