【摘要】：目的建立HPLC法測定龍膽藥材(飲片)中龍膽苦苷和落干酸的方法,提升龍膽藥材(飲片)質(zhì)量標準。方法 Fortis Xi-C18色譜柱(250 mm×4.0 mm,5μm),以乙腈-0.1%磷酸梯度為流動相(0→30 min,8:92→20:80),流速1.0 ml·min-1,檢測波長240 nm,柱溫25℃。結(jié)果龍膽苦苷和落干酸分別在10.02~501.00 mg·L-1和1.01~160.96 mg·L-1內(nèi)線性關(guān)系良好,平均回收率分別為100.2%(RSD為1.51%,n=9)和98.55%(RSD為2.26%,n=9)。所測定39批樣品中,堅龍膽中龍膽苦苷含量范圍為1.40%~4.82%,落干酸含量范圍為0.50%~1.06%;龍膽中龍膽苦苷含量范圍為2.35%~4.21%,落干酸含量范圍為0.96%~2.76%。結(jié)論該方法簡單,準確,靈敏,可靠,重現(xiàn)性好,可用于龍膽藥材(飲片)質(zhì)量控制研究。
[Abstract]:Objective to establish a HPLC method for the determination of gentiopicrin and lophanic acid in Gentian (Radix Gentianae) and to improve the quality standard of Radix Gentiana (Radix Gentianae). Methods the Fortis Xi-C18 column (250 mm 脳 4.0 mm, 5 渭 m) was used, the mobile phase was acetonitrile-0.1% phosphoric acid gradient (0 脳 30 min,8:92 路min-1), the flow rate was 1.0 ml 路min-1, and the detection wavelength was 240 nm, column temperature at 25 鈩，

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