【摘要】：白屈菜紅堿(CHE)是一種生物活性豐富的生物堿,可抗癌、殺菌,并具有良好的滅螺特性,但在植物體內含量較少、分離困難,因此它的高效分離一直備受人們的關注。本文合成了一種新型的核-殼-殼結構的分子印跡磁性聚合物微球,研究了該微球對白屈菜紅堿的吸附動力學與熱力學,并進行了白屈菜紅堿的高效分離研究。本文還深入探討了白屈菜紅堿與螺類功能蛋白——鑰孔戚血藍蛋白(KLH)的相互作用機理,為白屈菜紅堿作為天然滅螺藥物的開發(fā)提供了有益的參考。具體研究內容如下:(1)以白屈菜紅堿為模板,四乙烯基吡啶為功能單體,Fe3O4為磁性基質,采用自由基聚合法合成了白屈菜紅堿分子印跡磁性聚合物微球(CHE-MMIPs),并對其進行了形貌表征。結果表明,該微球具有較窄的粒徑分布(約240nm),良好的磁響應度(40emu/g),較好的單分散性。(2)考察了CHE-MMIPs的吸附效率。結果表明,CHE-MMIPs最大的吸附容量為12mg/g(6 h),吸附過程符合Langmuir等溫吸附模型及準二級動力學方程,屬單層吸附。(3)利用上述實驗純化的CHE,采用熒光光譜與分子對接技術研究了CHE對KLH的熒光猝滅機制。結果表明,CHE可以顯著猝滅KLH的熒光,其猝滅機制為靜態(tài)猝滅。在293K、298K、303K時,CHE與KLH的結合常數(shù)分別為4.72×105、5.06×105、1.01×106L/mol。體系的熱力學參數(shù)ΔG298K、ΔH298K、ΔS298K的值分別為 32.54 kJ/mol、 58.78 kJ/mol、和 88.05 J/mol·K。在結合過程中主要的作用力為范德華力和氫鍵。采用分子對接技術獲得了CHE與KLH結合的具體位置、位點數(shù)及結合親和力的大小。發(fā)現(xiàn)KLH中的Cu位點并不是CHE與KLH的潛在結合位點。
[Abstract]:(CHE) is a kind of alkaloid with rich biological activity, it can be anticancer, bactericidal and has good snail killing characteristics. However, it has little content in plants and is difficult to be separated. Therefore, its high efficiency separation has been paid close attention by people all the time. In this paper, a novel core-shell structure molecularly imprinted magnetic polymer microspheres was synthesized. The adsorption kinetics and thermodynamics of the microspheres for chordythrine were studied, and the high efficiency separation of alchlor alkaloids was studied. The mechanism of interaction between chordythrine and keyhole hemocyanin (KLH), a functional protein of snail, was also discussed in this paper, which provided a useful reference for the development of chordythrine as a natural snail killing drug. The main contents are as follows: (1) the molecular imprinted magnetic polymer microspheres (CHE-MMIPs) were synthesized by free radical polymerization using tartarine as template, tetravinylpyridine as functional monomer and Fe3O4 as magnetic matrix. Its morphology was characterized. The results showed that the microspheres had narrow particle size distribution (about 240nm), good magnetic responsivity (40emu/g) and good monodispersity. (2) the adsorption efficiency of CHE-MMIPs was investigated. The results show that the maximum adsorption capacity of CHE-MMIPs is 12mg/g (6-h), adsorption process accords with Langmuir isotherm adsorption model and quasi-second order kinetic equation, and belongs to monolayer adsorption. (3) the CHE, purified from the above experiments belongs to monolayer adsorption. The fluorescence quenching mechanism of CHE on KLH was studied by fluorescence spectroscopy and molecular docking technique. The results showed that CHE could significantly quench the fluorescence of KLH, and its quenching mechanism was static quenching. At 293K, 298K and 303K, the binding constants of CHE and KLH were 4.72 脳 105,5.06 脳 105,1.01 脳 106L mol. The thermodynamic parameters 螖 G298K, 螖 H298K and 螖 S298K are 32.54 kJ/mol, / mol 58.78 kJ/mol, and 88.05 J / mol 路K. The main forces in the bonding process are van der Waals force and hydrogen bond. The binding position, the number of sites and the binding affinity of CHE and KLH were obtained by molecular docking technique. It was found that the Cu site in KLH was not a potential binding site between CHE and KLH.
【學位授予單位】：湖南理工學院
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：O631.3

【參考文獻】

相關期刊論文 前10條

1 張秀梅;李成華;;脂多糖誘導的腫瘤壞死因子(LITAF)生物學功能研究進展[J];中國細胞生物學學報;2015年04期

2 唐萬俠;吳曉鋒;高萬壽;李桂芹;齊海燕;張樹軍;;白屈菜提取液的殺蟲活性研究[J];齊齊哈爾大學學報(自然科學版);2015年02期

3 王朝元;童勝蘭;胡鑫;;博落回生物堿成分及其抗菌活性的研究[J];中南民族大學學報(自然科學版);2015年01期

4 朱麗麗;曹玉華;曹光群;;核殼結構的雙酚A磁性納米分子印跡聚合物的合成及應用[J];分析化學;2013年11期

5 王培卿;尹震花;康文藝;;白屈菜紅堿藥理活性研究進展[J];中國中藥雜志;2013年17期

6 蘇立強;韓宇;溫雨;寧振鑫;;磁性胰蛋白酶分子印跡聚合物的制備及性能評價[J];化學研究與應用;2013年04期

7 王飛;戴建榮;;氯硝柳胺的毒理學安全性評價研究概況[J];中國人獸共患病學報;2013年01期

8 李丹;徐麗;張震;王育鵬;;喜旱蓮子草的利用及其生物防治研究進展[J];中國農學通報;2013年01期

9 梁興泉;劉舉;林寶鳳;黎演明;侯長軍;;四氧化三鐵存在下LDPE熱分解動力學研究[J];應用化工;2012年06期

10 呂慶榮;方慶清;劉艷美;;溶劑熱法合成納米結構四氧化三鐵空心微球磁性研究[J];人工晶體學報;2011年06期

相關碩士學位論文 前1條

1 趙嬌嬌;釘螺血藍蛋白功能域的克隆與重組表達研究[D];贛南師范學院;2013年

，

本文編號：2432858