【摘要】：目的:建立高效液相色譜法同時測定甜葉菊葉中新綠原酸、綠原酸、隱綠原酸、異綠原酸B、異綠原酸A和異綠原酸C6個酚酸類成分。方法:采用C18色譜柱(4.6 mm×250 mm,5μm),流動相為0.1%甲酸水溶液-乙腈,梯度洗脫,柱溫30℃,檢測波長327 nm。結(jié)果:6個酚酸質(zhì)量濃度在10~196μg·m L-1范圍內(nèi)線性關(guān)系良好;6個酚酸的加樣回收率分別為98.3%、95.2%、100.1%、97.3%、95.6%和96.1%,加樣回收率、精密度、重復(fù)性、穩(wěn)定性均符合有關(guān)規(guī)定;9批樣品中6個酚酸類成分的含量分別為新綠原酸0.062%~0.725%,綠原酸0.277%~2.450%,隱綠原酸0.096%~0.694%,異綠原酸B 0.046%~0.867%,異綠原酸A0.157%~0.772%,異綠原酸C 0.135%~1.821%,6個酚酸之和在1.182%~6.668%。結(jié)論:該方法可為甜葉菊進一步開發(fā)利用提供質(zhì)量控制方法。
[Abstract]:Objective: to establish a high performance liquid chromatography (HPLC) method for the simultaneous determination of 6 phenolic acids in stevia leaves such as Neo-Lv Yuan Acid, Cryptom_person1# Acid, Isobalipironic Acid B, IsoLv Yuan Acid A and isoLv Yuan Acid C6. Methods: C18 column (4.6 mm 脳 250 mm,5 渭 m),) was used as mobile phase of 0.1% formic acid-acetonitrile, gradient elution, column temperature 30 鈩，

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