【摘要】：使用溶劑誘導(dǎo)相變萃取對(duì)中成藥及原料藥進(jìn)行預(yù)處理,建立了中成藥中香豆素的超高效合相色譜(UPC2)檢測(cè)方法。樣品經(jīng)粉碎(60目)、乙腈-水(7∶3,體積比)提取,二氯甲烷誘導(dǎo)分相后,使用UPC2對(duì)樣品中的香豆素進(jìn)行檢測(cè)。以3倍信噪比(S/N)確定香豆素的檢出限為0.1 mg/L;線性范圍為0.3~10.0mg/L;加標(biāo)回收率為89.8%~102.3%;相對(duì)標(biāo)準(zhǔn)偏差為0.31%~1.0%。同時(shí)與UPLC進(jìn)行比較,結(jié)果表明該方法的選擇性高,檢測(cè)成本低,且分析時(shí)間僅為UPLC的1/4。該研究為高通量檢測(cè)香豆素提供了一種全新的前處理及檢測(cè)方法。
[Abstract]:Solvent-induced phase change extraction (SPE) was used to pretreat Chinese patent medicine and raw material, and a method for the determination of coumarin in Chinese patent medicine by ultra-high performance phase chromatography (UPC2) was established. The samples were pulverized (60 mesh), acetonitrile-water (7: 3, volume ratio) was extracted, dichloromethane induced phase separation, and coumarin in the sample was determined by UPC2. The detection limit of coumarin is 0. 1 mg/L;, the linear range is 0. 310. 0 mg / L, the recovery rate is 89. 8% and the relative standard deviation is 0. 31% and 1. 0%, respectively. Compared with UPLC, the results show that the method has high selectivity and low detection cost, and the analysis time is only 1 / 4 of that of UPLC. This study provides a new pretreatment and detection method for high-throughput coumarin detection.
【作者單位】： 甘肅出入境檢驗(yàn)檢疫局綜合技術(shù)中心;甘肅省檢驗(yàn)檢疫科學(xué)技術(shù)研究院;
【分類號(hào)】：O657.7;TQ460.72
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本文編號(hào)：2313380