【摘要】：通過化學分析和生物活性評價考察丹參藥材的品質差異,探討丹參抗血小板聚集生物活性的主要貢獻成分。采用高效液相色譜(HPLC)技術建立丹參藥材HPLC指紋圖譜,以抗血小板聚集相對效價作為指標,評價不同產地不同批次丹參藥材的品質差異,構建基于化學表征及生物效價測定的評價模式。結果表明,不同批次丹參藥材的HPLC指紋圖譜相似度很高(相似度0.930~0.998),而其抗血小板聚集相對效價相差10倍,提示化學指紋圖譜難以反映丹參的活性和質量差異。通過化學指紋圖譜與抗血小板聚集生物效價進行譜效相關分析,篩選出與生物活性相關系數大于0.5的6個色譜峰:二氫丹參酮Ⅰ、隱丹參酮、丹參酮Ⅰ、丹參酮ⅡA及2個未知化合物。對上述4種已知化合物單體進行活性驗證發(fā)現,隱丹參酮的抗血小板聚集活性最強,而其它3種丹參酮類化合物幾乎沒有體外抗血小板聚集活性。進一步比較丹參中高含量成分丹酚酸B與低含量成分隱丹參酮的活性貢獻,結果表明,兩者的活性貢獻基本相當,說明隱丹參酮是丹參中低含量高活性成分,對評價丹參質量具有重要貢獻度。
[Abstract]:The quality difference of Salvia miltiorrhiza was investigated by chemical analysis and bioactivity evaluation, and the main contribution components of Salvia miltiorrhiza against platelet aggregation were discussed. The HPLC fingerprint of Salvia miltiorrhiza was established by (HPLC), and the quality of different batches of Salvia miltiorrhiza was evaluated by using the relative titer of anti-platelet aggregation as an index. An evaluation model based on chemical characterization and bioassay was established. The results showed that the HPLC fingerprint of different batches of Salvia miltiorrhiza had high similarity (0.930 ~ 0.998), but the relative titer of anti-platelet aggregation was 10 times different, suggesting that the chemical fingerprint could not reflect the difference of activity and quality of Salvia miltiorrhiza. Six chromatographic peaks, dihydrotanshinone 鈪，

本文編號：2272556