【摘要】：超分子相互作用和形成共價(jià)鍵是進(jìn)行自組裝的主要手段和方法。利用分子間弱的相互作用力是超分子組裝的根本,可以實(shí)現(xiàn)大量構(gòu)筑基元在溫和條件下進(jìn)行簡(jiǎn)單快速,厚度、形貌、成分可控的平行組裝,為超分子組裝邁向工業(yè)化奠定了基礎(chǔ)。但是超分子組裝仍存在組裝力小、主客體分子生物毒性大、不同超分子力間相互干擾等缺點(diǎn),所以我們分別選擇生物相容性的Biotin/Avid in系統(tǒng)、可逆的S-S共價(jià)鍵氧化還原反應(yīng)作為宏觀自組裝的作用力。生物素/親和素是已知作用力最強(qiáng)的非共價(jià)鍵,并且特異性強(qiáng)、生物相容性好,可以運(yùn)用到生物相容性組織工程直接的構(gòu)筑上;S-S可以在不同的條件下進(jìn)行氧化還原反應(yīng),實(shí)現(xiàn)宏觀物體可逆的組裝/解組裝,其特點(diǎn)就是利用共價(jià)鍵作用力強(qiáng)實(shí)現(xiàn)更穩(wěn)定組裝體,并且我們使用帶SH的半胱氨酸作為功能分子,本身具有很好的生物相容性,可以為生物相容性組織工程支架的構(gòu)筑提供新的方法。我們首先制作PDMS小塊作為自組裝的宏觀基元,利用酰胺化接枝反應(yīng)在EDC/NHS的活化催化下于水相中分別合成了PAH-Biotin、PAH-SH等帶有特定官能團(tuán)的聚電解質(zhì),通過(guò)Layer-by-Layer方法修飾到PDMS小塊表面,使之帶有不同的組裝官能團(tuán),在特定的條件下實(shí)現(xiàn)宏觀基元的自組裝。為實(shí)現(xiàn)生物相容性的、可逆的、穩(wěn)定的、高選擇性的宏觀自組裝提供了一些新的研究方向。
[Abstract]:Supramolecular interaction and formation of covalent bonds are the main means and methods for self-assembly. The use of weak intermolecular interaction is fundamental to the supramolecular assembly, which can realize a large number of elements to be constructed in a simple, rapid, thickness, morphology, and controllable composition parallel assembly under mild conditions. It lays the foundation for supramolecular assembly to industrialization. However, supramolecular assembly still has some disadvantages, such as low assembly power, high toxicity of host and guest molecules, interference between different supramolecular forces, so we choose biocompatible Biotin/Avid in system. The reversible S S covalent redox reaction acts as a macro self assembly force. Biotin / avidin is a non-covalent bond with the strongest known force, and it has strong specificity and good biocompatibility, which can be used in direct construction of biocompatibility tissue engineering. S-S can be redox under different conditions. To achieve reversible assembly / unassembly of macroscopic objects, it is characterized by the use of covalent bond forces to achieve a more stable assembly, and we use cysteine with SH as functional molecules with good biocompatibility. It can provide a new method for the construction of biocompatibility tissue engineering scaffolds. In this paper, we first prepared PDMS fragments as macroelements for self-assembly. Polyelectrolytes such as PAH-Biotin,PAH-SH with specific functional groups were synthesized in aqueous phase by amidation graft reaction under the catalysis of EDC/NHS activation, and then modified to the surface of PDMS fragments by Layer-by-Layer method. It has different assembly functional groups and realizes the self-assembly of macro-elements under certain conditions. It provides some new research directions for achieving biocompatible, reversible, stable and highly selective macro-self-assembly.
【學(xué)位授予單位】：北京化工大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2016
【分類號(hào)】：O641.3

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本文編號(hào)：2266082