【摘要】：目的:建立清喉利咽顆粒的超高效液相色譜(UPLC)指紋圖譜,并結合主成分分析法(PCA)為其質量控制提供參考。方法:采用UPLC法。色譜柱為ACQUITY UPLC BEH C18,流動相為乙腈(A)-0.1%磷酸水溶液(B)(梯度洗脫),流速為0.4 m L/min,檢測波長為280 nm,柱溫為30℃,進樣量為2μL。以鞣花酸為參照物,分析12批清喉利咽顆粒樣品,采用"中藥指紋圖譜相似度評價系統(tǒng)"軟件進行相似度分析,并根據(jù)對照品對其中的共有峰進行指認,同時對共有峰進行主成分分析。結果:12批清喉利咽顆粒指紋圖譜標定了18個共有峰,指認了6個主要色譜峰(沒食子酸、鞣花酸、柚皮苷、橙皮苷、新橙皮苷、黃芩苷);12批樣品與對照指紋圖譜相似度均≥0.984。經(jīng)主成分分析,18個共有峰可綜合為3個主成分,累計方差貢獻率為78.277%;黃芩苷和16號峰為清喉利咽顆粒指紋圖譜中影響比較大的指標。結論:該方法可為清喉利咽顆粒的質量評價提供參考。
[Abstract]:Objective: to establish the (UPLC) fingerprint of Qinghaliyan granules by ultra-high performance liquid chromatography (HPLC), and to provide reference for its quality control with principal component analysis (PCA). Methods: UPLC method was used. The chromatographic column was ACQUITY UPLC BEH C18, the mobile phase was (B) (gradient elution of acetonitrile (A) -0.1% phosphoric acid solution), the flow rate was 0.4 mL / min, the detection wavelength was 30 鈩，

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