本文選題：組合式高分辨質(zhì)譜儀(LTQ + Orbitrap�。� 參考：《質(zhì)譜學(xué)報(bào)》2017年05期

【摘要】：利用組合式高分辨質(zhì)譜儀(LTQ Orbitrap XL)對(duì)均一褐藻膠寡糖,包括甘露糖醛酸寡糖和古洛糖醛酸寡糖進(jìn)行二級(jí)質(zhì)譜分析,比較了常規(guī)的碰撞誘導(dǎo)解離(CID)和高能碰撞解離(HCD)在寡糖結(jié)構(gòu)分析中的差異,并應(yīng)用HCD技術(shù)分析甘露糖醛酸寡糖和古洛糖醛酸寡糖的結(jié)構(gòu)差異。研究發(fā)現(xiàn),HCD可避免CID中的1/3效應(yīng),而且可以提供更多的糖環(huán)斷裂碎片信息,如~(2,5)A_2(m/z 291)、~(2,4)A(m/z 235、411)、~(2,5)A脫水(m/z101、277、453)以及Z2脫羧碎片(m/z307、483、659)等,進(jìn)一步驗(yàn)證了Z2脫羧碎片是區(qū)別甘露糖醛酸與古洛糖醛酸的特征碎片。同時(shí),HCD能夠提供豐富的裂解碎片信息,這為褐藻膠寡糖的結(jié)構(gòu)解析提供了依據(jù)。
[Abstract]:Combined high resolution mass spectrometer (LTQ Orbitrap XL) was used to analyze homogenous alginate oligosaccharides, including mannouronic acid oligosaccharides and gulouronic acid oligosaccharides. The differences between conventional collision induced dissociation (CID) and high energy collision dissociation (HCD) in the analysis of oligosaccharide structure were compared, and the structural differences between mannouronic acid oligosaccharides and gulouronic acid oligosaccharides were analyzed by HCD technique. It has been found that HCD can avoid the 1 / 3 effect in CID, and can provide more information on the breaking fragments of sugar ring, such as ~ (2 / 5) A _ 2 (m / z 291) ~ (2 + 4) A (/ m / z 235411) ~ (2 / 5) A dehydration (m/z101277453) and Z _ 2 decarboxylation fragment (m/z307483659), etc. It is further proved that Z 2 decarboxylation fragment is the characteristic fragment distinguishing mannouronic acid from gulouronic acid. At the same time, HCD can provide abundant information of pyrolysis fragments, which provides a basis for the structural analysis of alginate oligosaccharides.
【作者單位】： 中國海洋大學(xué)醫(yī)藥學(xué)院海洋藥物教育部重點(diǎn)實(shí)驗(yàn)室;
【基金】：中央高�；究蒲袠I(yè)務(wù)費(fèi)實(shí)驗(yàn)室基金項(xiàng)目(201551012)資助
【分類號(hào)】：O629.12;O657.63

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本文編號(hào)：2083513