本文選題：多肽組 + 納升液相色譜-高分辨串聯(lián)質(zhì)譜��； 參考：《分析化學(xué)》2016年05期

【摘要】：多肽組是指生物體表達(dá)的所有多肽,尿液等體液的多肽組是生物標(biāo)記物的重要來(lái)源。本研究采用氧化石墨烯-磷酸鑭納米磁性復(fù)合材料分離富集尿液多肽,進(jìn)行納升液相色譜-高分辨串聯(lián)質(zhì)譜分析,從單一樣本中鑒定了歸屬于123個(gè)蛋白質(zhì)的790條肽段。研究表明,這些肽段在蛋白質(zhì)水平上不是平均分布的。此外,本研究檢測(cè)到了肽段的氧化、磷酸化和脫氨化等翻譯后修飾現(xiàn)象,觀察到了尿液多肽的階梯序列性,從蛋白水平上對(duì)尿液多肽組進(jìn)行了生物信息學(xué)分析,結(jié)果表明,這些多肽所歸屬的大部分蛋白質(zhì)之間存在相互作用。本方法可為在尿液中尋找疾病的標(biāo)志物提供方法學(xué)支持。
[Abstract]:Polypeptide group refers to all polypeptides expressed by the organism. The polypeptide group of urine and other body fluids is an important source of biomarkers. This study uses the lanthanum oxide nano magnetic composite material to separate and enrich the urine polypeptide by using the lanthanum phosphate nanocomposite material, and carries out the nliter HPLC high resolution tandem mass spectrometry analysis, and identifies 123 proteins in a single sample. The study showed that the peptide segments were not evenly distributed at the protein level. In addition, this study detected the post-translational modification of peptide oxidation, phosphorylation and deamination, observed the step sequence of the urine polypeptide. The bioinformatics analysis of the peptide group in the urine was carried out from the protein level. The results showed that the peptide group was analyzed by bioinformatics from the protein level. The interactions between these peptides belong to most proteins. This method can provide methodological support for searching for markers of disease in urine.
【作者單位】： 深圳大學(xué)生命與海洋科學(xué)學(xué)院深圳市微生物基因工程重點(diǎn)實(shí)驗(yàn)室;深圳大學(xué)生命與海洋科學(xué)學(xué)院深圳市海洋生物資源與生態(tài)環(huán)境重點(diǎn)實(shí)驗(yàn)室;
【基金】：國(guó)家自然科學(xué)基金項(xiàng)目(No.31470804) 深圳市重點(diǎn)項(xiàng)目(No.JSGG20140703163838793) 深圳市基礎(chǔ)科學(xué)布局項(xiàng)目阿爾茨海默癥臨床診斷研究(No.基20150006)資助~~
【分類號(hào)】：R446.12;O657.63
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本文編號(hào)：1975222