發(fā)布時(shí)間：2018-05-31 06:52

  本文選題：木豆 + 葉��； 參考：《中成藥》2017年07期

【摘要】：目的建立木豆Cajanus cajan(L.)Millsp.葉HPLC指紋圖譜,并測定葒草苷和木犀草素的含有量。方法木豆葉65%甲醇提取液的分析采用Agilent Zorbax SB-C18色譜柱(4.6 mm×250 mm,5μm);以甲醇-1%醋酸為流動相,梯度洗脫;體積流量1.0 m L/min;柱溫25℃;檢測波長260 nm。結(jié)果 10批樣品(S1~S10)HPLC指紋圖譜中有21個(gè)共有峰,除S3(0.516)外,其相似度均大于0.950。葒草苷和木犀草素分別在0.089 5~3.960μg和0.015 5~0.408μg范圍內(nèi)呈良好的線性關(guān)系,平均加樣回收率分別為99.43%(RSD=1.32%)和98.50%(RSD=0.82%),3個(gè)產(chǎn)地(廣東、云南、海南)樣品中2種成分含有量有明顯差異。結(jié)論該方法簡單準(zhǔn)確,重復(fù)性好,可用于木豆葉的質(zhì)量控制。
[Abstract]:Objective to establish Cajanus cajanus L. Millsp. Leaf HPLC fingerprint and determination of orientin and luteolin content. Methods Agilent Zorbax SB-C18 column was used to analyze the 65% methanol extract from xylose leaves, the mobile phase was methanol-1% acetic acid as the mobile phase, the volume flow rate was 1.0 mL / min, the column temperature was 25 鈩，

本文編號：1958715