本文選題：竹茹 + 多糖��； 參考：《中國食品學報》2017年07期

【摘要】：目的:研究竹茹多糖(BSP)的結構特性及體外免疫調節(jié)活性。方法:利用甲基化分析及核磁共振波譜法解析BSP的化學結構;采用小鼠脾臟淋巴細胞轉化增殖試驗,RAW264.7巨噬細胞吞噬中性紅以及分泌NO試驗評價BSP的體外免疫調節(jié)活性。結果:BSP為乙酰化的阿拉伯糖基4-O-甲基葡萄糖醛酸木聚糖,其結構為:以β-1,4-D-木糖為骨架,在木糖基的C-2位上連接有乙�；�4-O-甲基-α-D-葡萄糖醛酸,C-3位上連接有乙酰基或α-L-阿拉伯糖;BSP在10~100μg/m L范圍內能促進刀豆蛋白或脂多糖誘導的小鼠脾臟淋巴細胞增殖;BSP在10~1 000μg/m L范圍可顯著提高RAW 264.7巨噬細胞吞噬中性紅及生成NO的能力,并呈明顯的劑量依賴性。結論:原料來源豐富的竹茹多糖具有作為天然免疫調節(jié)劑的潛力。
[Abstract]:Objective: to study the structure and immunomodulatory activity of BSPs. Methods: the chemical structure of BSP was analyzed by methylation analysis and nuclear magnetic resonance spectroscopy (NMR), the phagocytosis of neutral red by RAW264.7 macrophages and the in vitro immunomodulatory activity of BSP were evaluated by mouse spleen lymphocyte transformation and proliferation test (RAW264.7). Results: BSP was acetylated arabinyl 4-Omethylglucuronic acid xylan, and its structure was as follows: 尾 -1N 4-D-xylose was used as skeleton. Ligation of acetyl or 4-Omethyl- 偽 -Dglucuronic acid C-3 on C-2 site of xylose group with acetyl group or 偽 -L-arabinose BSP on C-3 site can promote proliferation of spleen lymphocytes induced by concanavalin or lipopolysaccharide in 10 ~ 100 渭 g / m L range. The ability of RAW 264.7 macrophages to phagocytosis of neutrophilic red and to produce no was significantly increased in the range of 10 ~ 1 000 渭 g 路mL ~ (-1) ~ (-1) 渭 g 路mL ~ (-1). And in a dose-dependent manner. Conclusion: the polysaccharide with abundant raw materials has the potential as a natural immunomodulator.
【作者單位】： 浙江大學生物系統(tǒng)工程與食品科學學院浙江省農(nóng)產(chǎn)品加工技術研究重點實驗室浙江省食品加工技術與裝備工程中心;
【分類號】：O629.12
，

本文編號：1921741