本文選題：復方板藍根顆粒 + 指紋圖譜�。� 參考：《質譜學報》2017年03期

【摘要】：建立了復方板藍根顆粒提取物的大氣壓化學電離質譜(APCI-MS)的指紋圖譜分析方法,并利用液相色譜-大氣壓化學電離-串聯(lián)質譜(LC-APCI-MS~n)技術對主要指紋峰的歸屬進行確認。結果表明:質譜指紋圖譜確定了10批不同來源復方板藍根顆粒提取物的10個共有峰,各提取物的指紋圖譜相似度均在97%以上�；瘜W成分的LC-APCI-MS~n分析鑒別了復方板藍根顆粒提取物中吲哚類、奎唑酮類、有機酸類和卟啉類等12個化合物,并確定了5個質譜指紋峰的歸屬。采用體外實驗研究對復方板藍根顆粒提取物的抗病毒活性進行評價,結果表明,其具有一定的抗病毒活性。該指紋圖譜的精密度、穩(wěn)定性和重現(xiàn)性良好,化學成分分析快捷、靈敏,結合化學成分分析和藥效學研究,可為復方板藍根顆粒質量控制標準的制訂奠定基礎,同時也為深入研究其藥效物質基礎和譜效關系提供理論依據。
[Abstract]:A fingerprint analysis method for APCI-MS of compound Isatis root granule extract was established. The main fingerprint peaks were confirmed by liquid chromatography-atmospheric pressure chemical ionization tandem mass spectrometry (LC-APCI-MSN) technique. The results showed that the 10 common peaks of 10 batches of compound Radix Isatidis granules extracts were identified by mass spectrometry, and the fingerprint similarity of each extract was more than 97%. LC-APCI-MS~n analysis of the chemical constituents identified 12 compounds such as indole, quezolidone, organic acids and porphyrins in compound Isatis root granule extracts, and determined the attribution of five MS fingerprint peaks. The antiviral activity of compound Radix Isatidis granules extract was evaluated in vitro. The results showed that it had certain antiviral activity. The fingerprint has good precision, stability and reproducibility, quick and sensitive chemical component analysis, combined with chemical composition analysis and pharmacodynamics study, can lay a foundation for the formulation of quality control standard of compound Isatis root granules. At the same time, it also provides the theoretical basis for the further study of the substance basis and spectrum effect relationship of its pharmacodynamics.
【作者單位】： 核工業(yè)北京地質研究院;中國科學院長春應用化學研究所;
【分類號】：O657.63;R286.0
，

本文編號：1885639