本文選題：亞臨界丁烷 + 阿維菌素　； 參考：《分析化學》2017年06期

【摘要】：以茶樹鮮葉為原料,利用亞臨界丁烷流體分離鮮葉表面殘留的阿維菌素。在料溶比為1∶10(m/V)的條件下,利用響應面法(RSM)的Box-Benhnken Design(BBD)設計實驗,考察不同溫度、時間、分離次數(shù)等條件對多酚氧化酶活性的影響,同時分析亞臨界丁烷處理對茶樹鮮葉生理特性的影響。結果表明,亞臨界丁烷流體在保持茶葉物理結構和多酚氧化酶活性基本不變的情況下,可有效分離茶葉中的阿維菌素;通過Design Expert軟件分析,得出分離阿維菌素的最優(yōu)工藝參數(shù)為溫度45℃,時間30 min,分離次數(shù)1次,料液比為1∶10(m/V),分離效率大于91%,多酚氧化酶相對活性為25.73%。通過掃描電鏡觀察,茶樹鮮葉結構在丁烷處理前后無明顯變化。本研究結果表明,亞臨界丁烷流體可有效分離茶葉中的農(nóng)藥殘留,為亞臨界流體在去除天然植物農(nóng)殘的應用中提供了實驗依據(jù)和技術支持。
[Abstract]:Fresh tea leaves were used to separate abamectin from fresh leaves by subcritical butane fluid. At the ratio of 1: 10 m / v), the experiment was designed by using the response surface method (RSM), and the effects of different temperature, time and separation times on the activity of polyphenol oxidase were investigated. The effects of subcritical butane treatment on the physiological characteristics of tea fresh leaves were also analyzed. The results showed that the subcritical butane fluid could effectively separate avermectin from tea leaves under the condition of keeping the physical structure of tea leaves and the activity of polyphenol oxidase unchanged, and could be analyzed by Design Expert software. The optimum technological parameters for separation of avermectin were as follows: temperature 45 鈩，

本文編號：1866333