本文選題：毛細(xì)管電泳 + 細(xì)菌纖維素　； 參考：《色譜》2017年12期

【摘要】：建立了高效毛細(xì)管電泳同時(shí)測(cè)定果蔬中3種常見(jiàn)的苯并咪唑類殺菌劑(甲基硫菌靈、多菌靈和苯菌靈)的分析方法。以細(xì)菌纖維素(BC)為電泳緩沖液添加劑來(lái)提高毛細(xì)管電泳的分離能力。系統(tǒng)考察了檢測(cè)波長(zhǎng)、緩沖液離子強(qiáng)度、緩沖液pH、分離電壓及BC添加量對(duì)3種苯并咪唑類殺菌劑分離效果的影響。最終的優(yōu)化條件:H3BO3/Na2B4O7緩沖液(4 mmol/L,pH 9.0);BC添加質(zhì)量分?jǐn)?shù)0.3%;運(yùn)行電壓15 kV;分離柱溫25℃;檢測(cè)波長(zhǎng)275 nm。3種苯并咪唑類殺菌劑在8 min之內(nèi)可實(shí)現(xiàn)基線分離及準(zhǔn)確定量。結(jié)果顯示:3種苯并咪唑類殺菌劑在各自線性范圍內(nèi)線性關(guān)系良好,相關(guān)系數(shù)(r2)≥0.997;檢出限為5.0~10.0μg/L;保留時(shí)間及峰面積的日間相對(duì)標(biāo)準(zhǔn)偏差(n=5)分別為0.82%~1.0%和2.4%~2.9%。該法用于葡萄、番茄及黃瓜中3種苯并咪唑類殺菌劑的測(cè)定,加標(biāo)回收率為93.5%~103.0%,RSD≤8.0%。該法可作為果蔬中甲基硫菌靈、多菌靈和苯菌靈同時(shí)檢測(cè)的有效手段。
[Abstract]:A method for simultaneous determination of benzimidazole fungicides (methyl thiamethyl, carbendazim and benzyl) in fruits and vegetables by high performance capillary electrophoresis (HPCE) was established. The separation ability of capillary electrophoresis was improved by using bacterial cellulose (BC) as buffer additive. The effects of detection wavelength, ionic strength of buffer, pH of buffer, separation voltage and addition of BC on the separation of three benzimidazole fungicides were systematically investigated. The optimized conditions were as follows: 4 mmol / L buffer solution of H _ 3BO _ 3 / Na _ 2B _ 4O _ 7, pH 9.0g / L, running voltage of 15 KV, column temperature of 25 鈩，

本文編號(hào)：1860030