本文選題：多級孔二氧化硅　切入點：葡萄糖傳感器　出處：《揚州大學(xué)》2016年碩士論文

【摘要】：第一部分基于微孔-大孔二氧化硅材料(HS)的葡萄糖傳感器的制備及應(yīng)用采用二元溶劑體系表面活性劑自組裝法,在水／乙醇二元溶劑體系中,以九水偏硅酸鈉為硅源,以陽離子表面活性劑十六烷基三甲基氯化銨為模板,制備出具有大孔和微孔的多級孔二氧化硅(HS)。并基于此材料構(gòu)建葡萄糖傳感器,探討了該葡萄糖傳感器的電化學(xué)性能以及對葡萄糖的檢測應(yīng)用。研究表明,該傳感器對葡萄糖有較好的電化學(xué)響應(yīng)、良好的重復(fù)性和穩(wěn)定性,其線性范圍為0.1-3.25 mM,最低檢出限為0.05 mM (S/N=3),靈敏度為1.81 mA-M-1·-cm-2。第二部分基于氨基化多級介孔二氧化硅材料(HPSNs-NH2)的葡萄糖傳感器的制備及應(yīng)用采用多元溶劑體系表面活性劑自組裝法,在水／乙醇／乙醚三元溶劑體系中,以硅酸四乙酯為硅源,以陽離子表面活性劑十六烷基三甲基溴化銨為模板劑,以3-氨丙基三乙氧基硅烷為硅烷偶聯(lián)劑,制備出氨基化多級介孔二氧化硅(HPSNs-NH2)。并基于此材料構(gòu)建葡萄糖傳感器,進一步探討該葡萄糖傳感器的電化學(xué)性能以及對葡萄糖的檢測應(yīng)用。研究表明,該傳感器對葡萄糖具有較好的電化學(xué)響應(yīng)能力且具有良好的重復(fù)性和穩(wěn)定性,對葡萄糖檢測的線性范圍為0.1-2.60 mM,最低檢出限為0.05 mM (S/N=3),靈敏度為3.95mA·M-1·cm-2。第三部分基于固載金納米顆粒的多級孔二氧化硅材料(HPSNs-Au)的葡萄糖傳感器的制備及應(yīng)用利用自組裝-靜電吸附法制備固載金納米顆粒的多級孔二氧化硅(HPSNs-Au),并基于此材料構(gòu)建葡萄糖傳感器。制備的材料具有較大的比表面積和良好的生物相容性,能為葡萄糖氧化酶提供良好的微環(huán)境；固載的金納米顆粒有利于提高電子給體與電子受體間的傳遞速率,增強酶膜的導(dǎo)電性。研究表明,該傳感器對葡萄糖具有快速的電化學(xué)響應(yīng)能力,對葡萄糖檢測的線性范圍為0.05-1.35 mM,最低檢出限為0.01mM(S/N=3),靈敏度為19.65mA·M-1·cm-2,且具有較高的選擇性以及良好的重復(fù)性和穩(wěn)定性。
[Abstract]:The first part is about the preparation and application of glucose sensor based on microporous and macroporous silica (HS2). In the water / ethanol binary solvent system, sodium metasilicate is used as silicon source. Using cationic surfactant cetyltrimethylammonium chloride as template, multiporous silica with macropore and micropore was prepared. Glucose sensor was constructed based on this material. The electrochemical performance of the glucose sensor and its application to glucose detection are discussed. The results show that the sensor has good electrochemical response, good reproducibility and stability to glucose. The linear range is 0.1-3.25 mm, the lowest detection limit is 0.05mM / s / N ~ (3 +), and the sensitivity is 1.81 mA-M-1 -cm-2.The second part is the preparation and application of a glucose sensor based on amino multistage mesoporous silica (HPSNs-NH _ 2) using a multi-component solvent system surfactant self-assembly method. In water / ethanol / ether ternary solvent system, tetraethyl silicate was used as silicon source, cationic surfactant cetyltrimethylammonium bromide as template and 3-aminopropyltriethoxysilane as silane coupling agent. An aminated multistage mesoporous silica HPSNs-NH _ 2 was prepared. Based on this material, a glucose sensor was constructed. The electrochemical properties of the glucose sensor and its application to glucose detection were investigated. The sensor has good electrochemical response to glucose and has good repeatability and stability. The linear range for glucose detection is 0.1-2.60 mm, the minimum detection limit is 0.05mm / s / n ~ (3) and the sensitivity is 3.95mA M ~ (-1) cm ~ (-2). The third part is the preparation and application of a glucose sensor based on multilevel porous silica material (HPSNs-Au) supported gold nanoparticles. -Electrostatic adsorption method was used to prepare multiporous silica loaded with gold nanoparticles, and glucose sensors were constructed on the basis of this material. The prepared materials have a large specific surface area and good biocompatibility. It can provide a good microenvironment for glucose oxidase, and the immobilized gold nanoparticles can improve the transfer rate between electron donor and electron receptor and enhance the conductivity of enzyme membrane. The sensor has a rapid electrochemical response to glucose. The linear range for glucose detection is 0.05-1.35 mm, the lowest detection limit is 0.01 mm / s / n ~ (-1), the sensitivity is 19.65mA M-1 / cm ~ (-2), and the sensor has high selectivity, good repeatability and stability.
【學(xué)位授予單位】：揚州大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2016
【分類號】：O657.1

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