本文選題：鳥槍法脂質(zhì)組學(xué)　切入點(diǎn)：多維質(zhì)譜　出處：《食品科學(xué)》2017年02期 　論文類型：期刊論文

【摘要】：建立以二醇基鍵合硅膠(Si O2/diol)為填料的固相萃取(solid-phase extraction,SPE)和基于多維度串聯(lián)質(zhì)譜的鳥槍法分離純化并測(cè)定烏鱧肌肉組織中的磷脂。利用Folch法和SPE法提取并純化磷脂,比較3種不同填料SPE柱的萃取效果,優(yōu)化SPE參數(shù),最佳條件:上樣量100?μL,淋洗液p H值為2,洗脫液為體積分?jǐn)?shù)50%乙腈溶液1 m L。所得提取物以直接進(jìn)樣的方法流動(dòng)注射入電噴霧離子源,經(jīng)三重四極桿質(zhì)譜的母離子掃描和中性丟失掃描模式,實(shí)現(xiàn)對(duì)磷脂分子種類的鑒定和定量。同時(shí),通過多維度串聯(lián)質(zhì)譜分析,得到各個(gè)磷脂分子中2條脂肪酸鏈長(zhǎng)度和不飽和度信息。實(shí)驗(yàn)結(jié)果顯示,烏鱧樣品中成功檢出磷脂酰膽堿、磷脂酰乙醇胺、磷脂酰肌醇和磷脂酰絲氨酸4類磷脂,共計(jì)67種磷脂分子,且大部分磷脂均含有不飽和脂肪酸鏈。本方法簡(jiǎn)單高效,分析準(zhǔn)確快捷,結(jié)果穩(wěn)定。
[Abstract]:Solid phase extraction (SPE) with diol bonded silica gel (Sio _ 2 / diol) as filler and bird gun method based on multi-dimensional tandem mass spectrometry were used to isolate and determine phospholipid in the muscle tissue of snakehead Argus Argus. Phospholipid was extracted and purified by Folch and SPE. The extraction efficiency of three different packing SPE columns was compared and the SPE parameters were optimized. The optimum conditions were as follows: sample amount 100? 渭 L, pH value of eluant was 2, elution solution was 50% acetonitrile solution 1 mL by volume fraction. The extract was injected into electrospray ion source by direct injection method, and was scanned by mother ion and neutral loss by triple quadrupole mass spectrometry. The identification and quantification of phospholipids were realized. At the same time, the length and unsaturation information of two fatty acid chains in each phospholipid molecule were obtained by multi-dimensional tandem mass spectrometry. Phosphatidylcholine, phosphatidylethanolamine, phosphatidylinositol and phosphatidyl serine were successfully detected in snakehead samples. The analysis is accurate and quick, and the result is stable.
【作者單位】： 浙江工商大學(xué)海洋食品研究院;
【基金】：國(guó)家國(guó)際科技合作專項(xiàng)(2014DFA32880) 國(guó)家自然科學(xué)基金青年科學(xué)基金項(xiàng)目(31601542) 浙江省自然科學(xué)基金項(xiàng)目(LQ16C200001)
【分類號(hào)】：TS254.7;O657.63

【參考文獻(xiàn)】

相關(guān)期刊論文 前2條

1 李婷婷;;鳥槍法快速分析魚貝類磷脂成分[J];食品科技;2015年07期

2 古再麗努爾·阿爾肯;劉然;侯俊峰;艾散江·艾海提;關(guān)明;;基于高效薄層色譜法的胡麻卵磷脂質(zhì)量分析[J];食品科學(xué);2014年16期

【共引文獻(xiàn)】

相關(guān)期刊論文 前2條

1 沈清;金仁耀;馮俊麗;薛靜;陳康;戴志遠(yuǎn);;二醇基硅膠固相萃取-多維質(zhì)譜對(duì)烏鱧組織脂質(zhì)組學(xué)分析[J];食品科學(xué);2017年02期

2 王迎春;鄭璐俠;許旭;陳鋼;陳桂良;楊新磊;;超臨界流體色譜法測(cè)定蛋黃卵磷脂中的8種磷脂組分[J];藥物分析雜志;2016年02期

【二級(jí)參考文獻(xiàn)】

相關(guān)期刊論文 前3條

1 郭R，

本文編號(hào)：1648641