本文選題：納米復(fù)合材料　切入點：光電化學(xué)生物傳感器　出處：《山東師范大學(xué)》2017年碩士論文　論文類型：學(xué)位論文

【摘要】：光電化學(xué)(PEC)檢測是一種新開發(fā)的適用于傳感檢測平臺的分析技術(shù),通過將光生載流子和電化學(xué)信號相結(jié)合,以檢測在光電極表面發(fā)生的化學(xué)反應(yīng)變化。光電化學(xué)生物傳感器已應(yīng)用于生物分析、臨床檢測、環(huán)境保護、食品檢驗等諸多領(lǐng)域。近年來,基于納米復(fù)合材料的PEC傳感器的研制及分析取得了突破性進展。本文中,利用半導(dǎo)體與二維納米材料的復(fù)合制備了新型PEC傳感器,基于納米復(fù)合材料具有大的比表面積、優(yōu)異的導(dǎo)電性能和較高的光電轉(zhuǎn)換效率,進行了以下工作內(nèi)容:1.通過一種簡單、有效的方法合成了WS2/CdS異質(zhì)結(jié)構(gòu)納米復(fù)合材料,并成功應(yīng)用于小分子的光電化學(xué)檢測。首先,通過電泳沉積的方法將用水/乙醇混合溶液分散的WS2納米片沉積在ITO電極上,隨后經(jīng)由簡單的連續(xù)離子層吸附反應(yīng)(SILAR)在WS2薄層上生長CdS納米顆粒�；谄洚愘|(zhì)結(jié)構(gòu)以及復(fù)合材料較大的比表面積、有效電荷轉(zhuǎn)移等,傳感器的光電性能有了實質(zhì)性的增強。L-半胱氨酸作為待測的生物小分子用于PEC生物傳感器的檢測,其檢測下限為5.29 nM;線性范圍跨度較大,為0.07μM至300μM,顯示出了修飾電極具有比較滿意的靈敏度、選擇性和的穩(wěn)定性。此外,結(jié)果表明此光電傳感器在實際樣品分析中具有良好的抗干擾能力和令人滿意的精確度,為光電生物傳感器在將來各領(lǐng)域中的應(yīng)用奠定了良好的基礎(chǔ)。2.通過在石墨烯量子點-二氧化鈦納米管(GQDs/TiO2 NTs)復(fù)合材料的基礎(chǔ)上連接用于識別目標分子的適配體DNA,建立了新型的超靈敏、無標記的PEC適配體傳感器。在該體系中,選擇氯霉素(CAP)作為目標分析物來展現(xiàn)光電化學(xué)適配體傳感器的分析性能�？箟难�(AA)在可見光照射下可有效清除復(fù)合納米材料的光生空穴,因此本體系選用它作為整個實驗的電子供體。另外,與TiO2 NTs的能級相比GQDs具有較高的LUMO和HOMO能級,二者的能級構(gòu)成階梯狀結(jié)構(gòu),有利于電子空穴的分離,表現(xiàn)為光電極的響應(yīng)顯著增強。氯霉素適配體通過π-π堆積作用固定在石墨烯量子點上,在最佳的條件下,光電化學(xué)適配體傳感器對氯霉素的檢測顯示了較低的檢測下限57.9 pM以及較寬的線性范圍0.5 nM-100 nM,同時該適配體傳感器也具備特異性、重現(xiàn)性和穩(wěn)定性良好的性質(zhì)。此光電化學(xué)檢測平臺為適配體傳感器的構(gòu)建提供了一種簡單、快速、廉價的策略,并且可以廣泛應(yīng)用于生物樣品中CAP殘留的靈敏檢測。
[Abstract]:Photochemical PEC-detection is a newly developed analytical technology suitable for sensor detection platform, which combines photogenerated carriers with electrochemical signals. Photochemical biosensor has been used in many fields such as biological analysis, clinical detection, environmental protection, food inspection and so on. The research and analysis of PEC sensors based on nanocomposites have made a breakthrough. In this paper, a new type of PEC sensor was prepared by using semiconductor and two-dimensional nano-materials, based on the large specific surface area of nanocomposites. With excellent conductivity and high photoelectric conversion efficiency, the following work has been done: 1. WS2/CdS heterostructure nanocomposites were synthesized by a simple and effective method, and successfully applied to photochemical detection of small molecules. The WS2 nanoparticles dispersed in water / ethanol solution were deposited on the ITO electrode by electrophoretic deposition. Then CdS nanoparticles were grown on WS2 thin layer by a simple continuous ion layer adsorption reaction (SILARR) based on its heterogeneity and the large specific surface area and effective charge transfer of the composite. The photoelectric performance of the sensor is substantially enhanced. L- cysteine is used as a biological small molecule to be measured for the detection of PEC biosensor with a detection limit of 5.29 nm. From 0.07 渭 M to 300 渭 M, it shows that the modified electrode has satisfactory sensitivity, selectivity and stability. In addition, the results show that the photoelectric sensor has good anti-interference ability and satisfactory accuracy in sample analysis. It lays a good foundation for the application of photoelectric biosensor in various fields in the future. 2. By joining the aptamer DNA to identify the target molecule on the basis of graphene quantum dot-titania nanotube / TiO2 nanotube composite material, the aptamer DNA of the target molecule is constructed. A new type of hypersensitivity, Unlabeled PEC adaptor sensors. In this system, Chloramphenicol (CAP) was selected as the target analyte to demonstrate the analytical performance of photochemical aptamer sensor. Ascorbic acid (AAA) can effectively remove the photogenerated holes of composite nanomaterials under visible light irradiation. In addition, compared with the energy level of TiO2 NTs, GQDs has higher energy levels of LUMO and HOMO, and their energy levels form a ladder structure, which is conducive to the separation of electron holes. Chloramphenicol aptamer is immobilized on graphene quantum dots by 蟺-蟺 stacking, under the optimum conditions, The detection of chloramphenicol by photochemical aptamer sensor showed a lower detection limit of 57.9 pm and a wide linear range of 0.5 nM-100 nm, and the aptamer sensor was also specific. This photochemical detection platform provides a simple, rapid and inexpensive strategy for the construction of aptamer sensors, and can be widely used in sensitive detection of CAP residues in biological samples.
【學(xué)位授予單位】：山東師范大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：TP212.3;O657

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本文編號：1593197